Effect of carbon addition and predation on acetate-assimilating bacterial cells in groundwater
Date
2009-07-13Author
Longnecker, Krista
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Da Costa, Andreia
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Bhatia, Maya P.
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Kujawinski, Elizabeth B.
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https://hdl.handle.net/1912/3851As published
https://doi.org/10.1111/j.1574-6941.2009.00767.xAbstract
Groundwater microbial community dynamics are poorly understood due to the
challenges associated with accessing subsurface environments. In particular, microbial
interactions and their impact on the subsurface carbon cycle remain unclear. In the
present project, stable isotope probing with uniformly-labeled [13C]-acetate was used to
identify metabolically-active and inactive bacterial populations based on their ability to
assimilate acetate and/or its metabolites. Furthermore, we assessed whether substrate
availability (bottom-up control) or grazing mortality (top-down control) played a greater
role in shaping bacterial community composition by separately manipulating the organic
carbon supply and the protozoan grazer population. A community fingerprinting
technique, Terminal Restriction Fragment Length Polymorphism (T-RFLP), revealed that
the bacterial community was not affected by changes in acetate availability but was
significantly altered by the removal of protozoan grazers. In silico identification of
terminal restriction fragments and 16S rDNA sequences from clone libraries revealed a
bacterial community dominated by Proteobacteria, Firmicutes, and Bacteroidetes.
Elucidation of the factors that structure the bacterial community will improve our
understanding of the bacterial role in the carbon cycle of this important subterranean
environment.
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Author Posting. © The Author(s), 2009. This is the author's version of the work. It is posted here by permission of Blackwell for personal use, not for redistribution. The definitive version was published in FEMS Microbiology Ecology 70 (2009): 456-470, doi:10.1111/j.1574-6941.2009.00767.x.
Suggested Citation
Preprint: Longnecker, Krista, Da Costa, Andreia, Bhatia, Maya P., Kujawinski, Elizabeth B., "Effect of carbon addition and predation on acetate-assimilating bacterial cells in groundwater", 2009-07-13, https://doi.org/10.1111/j.1574-6941.2009.00767.x, https://hdl.handle.net/1912/3851Related items
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