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    Effect of carbon addition and predation on acetate-assimilating bacterial cells in groundwater

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    LongneckerEtAl.FEMS.07.13.2009_with-figs.pdf (293.0Kb)
    Date
    2009-07-13
    Author
    Longnecker, Krista  Concept link
    Da Costa, Andreia  Concept link
    Bhatia, Maya P.  Concept link
    Kujawinski, Elizabeth B.  Concept link
    Metadata
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    Citable URI
    https://hdl.handle.net/1912/3851
    As published
    https://doi.org/10.1111/j.1574-6941.2009.00767.x
    Keyword
     Groundwater microbiology; Stable-isotope probing; Microbial community structure 
    Abstract
    Groundwater microbial community dynamics are poorly understood due to the challenges associated with accessing subsurface environments. In particular, microbial interactions and their impact on the subsurface carbon cycle remain unclear. In the present project, stable isotope probing with uniformly-labeled [13C]-acetate was used to identify metabolically-active and inactive bacterial populations based on their ability to assimilate acetate and/or its metabolites. Furthermore, we assessed whether substrate availability (bottom-up control) or grazing mortality (top-down control) played a greater role in shaping bacterial community composition by separately manipulating the organic carbon supply and the protozoan grazer population. A community fingerprinting technique, Terminal Restriction Fragment Length Polymorphism (T-RFLP), revealed that the bacterial community was not affected by changes in acetate availability but was significantly altered by the removal of protozoan grazers. In silico identification of terminal restriction fragments and 16S rDNA sequences from clone libraries revealed a bacterial community dominated by Proteobacteria, Firmicutes, and Bacteroidetes. Elucidation of the factors that structure the bacterial community will improve our understanding of the bacterial role in the carbon cycle of this important subterranean environment.
    Description
    Author Posting. © The Author(s), 2009. This is the author's version of the work. It is posted here by permission of Blackwell for personal use, not for redistribution. The definitive version was published in FEMS Microbiology Ecology 70 (2009): 456-470, doi:10.1111/j.1574-6941.2009.00767.x.
    Collections
    • Geology and Geophysics (G&G)
    • Marine Chemistry and Geochemistry (MC&G)
    Suggested Citation
    Preprint: Longnecker, Krista, Da Costa, Andreia, Bhatia, Maya P., Kujawinski, Elizabeth B., "Effect of carbon addition and predation on acetate-assimilating bacterial cells in groundwater", 2009-07-13, https://doi.org/10.1111/j.1574-6941.2009.00767.x, https://hdl.handle.net/1912/3851
     

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