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dc.contributor.authorWhalen, Kristen E.  Concept link
dc.contributor.authorSotka, Erik E.  Concept link
dc.contributor.authorGoldstone, Jared V.  Concept link
dc.contributor.authorHahn, Mark E.  Concept link
dc.date.accessioned2010-08-11T19:34:43Z
dc.date.available2010-08-11T19:34:43Z
dc.date.issued2010-05-14
dc.identifier.urihttps://hdl.handle.net/1912/3845
dc.descriptionAuthor Posting. © The Author(s), 2010. This is the author's version of the work. It is posted here by permission of Elsevier B.V. for personal use, not for redistribution. The definitive version was published in Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology 152 (2010): 288-300, doi:10.1016/j.cbpc.2010.05.003.en_US
dc.description.abstractMultixenobiotic transporters have been extensively studied for their ability to modulate the disposition and toxicity of pharmacological agents, yet their influence in regulating the levels of dietary toxins within marine consumers has only recently been explored. This study presents functional and molecular evidence for multixenobiotic transporter-mediated efflux activity and expression in the generalist gastropod Cyphoma gibbosum, and the specialist nudibranch Tritonia hamnerorum, obligate predators of chemically defended gorgonian corals. Immunochemical analysis revealed that proteins with homology to permeability glycoprotein (P-gp) were highly expressed in T. hamnerorum whole animal homogenates and localized to the apical tips of the gut epithelium, a location consistent with a role in protection against ingested prey toxins. In vivo dye assays with specific inhibitors of efflux transporters demonstrated the activity of P-gp and multidrug resistance-associated protein (MRP) families of ABC transporters in T. hamnerorum. In addition, we identified eight partial cDNA sequences encoding two ABCB and two ABCC proteins from each molluscan species. Digestive gland transcripts of C. gibbosum MRP-1, which have homology to vertebrate glutathione-conjugate transporters, were constitutively expressed regardless of gorgonian diet. This constitutive expression may reflect the ubiquitous presence of high affinity substrates for C. gibbosum glutathione transferases in gorgonian tissues likely necessitating export by MRPs. Our results suggest that differences in multixenobiotic transporter expression patterns and activity in molluscan predators may stem from the divergent foraging strategies of each consumer.en_US
dc.description.sponsorshipFinancial support was provided by the Ocean Life Institute Tropical Research Initiative Grant (WHOI) to KEW and MEH; the Robert H. Cole Endowed Ocean Ventures Fund (WHOI) to KEW; the National Undersea Research Center – Program Development Proposal (CMRC-03PRMN0103A) to KEW; and the National Science Foundation (Graduate Research Fellowship to KEW and DEB-0919064 to EES).en_US
dc.format.mimetypeapplication/pdf
dc.language.isoen_USen_US
dc.relation.urihttps://doi.org/10.1016/j.cbpc.2010.05.003
dc.subjectABC transporteren_US
dc.subjectAllelochemicalen_US
dc.subjectCalcein-amen_US
dc.subjectGorgonianen_US
dc.subjectMK571en_US
dc.subjectMRPen_US
dc.subjectP-gpen_US
dc.subjectVerapamilen_US
dc.titleThe role of multixenobiotic transporters in predatory marine molluscs as counter-defense mechanisms against dietary allelochemicalsen_US
dc.typePreprinten_US


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