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    Optical analysis of circuitry for respiratory rhythm in isolated brainstem of foetal mice

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    Muller et al mss Proc Roy Soc 09.pdf (838.3Kb)
    Date
    2008-09
    Author
    Muller, Kenneth J.  Concept link
    Tsechpenakis, Gavriil  Concept link
    Homma, Ryota  Concept link
    Nicholls, John G.  Concept link
    Cohen, Lawrence B.  Concept link
    Eugenin, Jaime  Concept link
    Metadata
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    Citable URI
    https://hdl.handle.net/1912/3843
    As published
    https://doi.org/10.1098/rstb.2009.0070
    Abstract
    Respiratory rhythms arise from neurons situated in the ventral medulla. We are investigating their spatial and functional relationships optically by measuring changes in intracellular calcium using the fluorescent, calcium-sensitive dye Oregon Green 488 BAPTA-1 AM while simultaneously recording the regular firing of motoneurons in the phrenic nerve in isolated brainstem/spinal cord preparations of E17 to E19 mice. Responses of identified cells are associated breath by breath with inspiratory and expiratory phases of respiration and depend on CO2 and pH levels. Optical methods including two-photon microscopy are being developed together with computational analyses. Analysis of the spatial pattern of neuronal activity associated with respiratory rhythm, including cross-correlation analysis, reveals a network distributed in the ventral medulla with intermingling of neurons that are active during separate phases of the rhythm. Our experiments, aimed at testing whether initiation of the respiratory rhythm depends on pacemaker neurons, on networks or a combination of both, suggest an important role for networks.
    Description
    Author Posting. © The Author(s), 2008. This is the author's version of the work. It is posted here by permission of Royal Society for personal use, not for redistribution. The definitive version was published in Philosophical Transactions of the Royal Society B Biological Sciences 364 (2009): 2485-2491, doi:10.1098/rstb.2009.0070.
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    Suggested Citation
    Preprint: Muller, Kenneth J., Tsechpenakis, Gavriil, Homma, Ryota, Nicholls, John G., Cohen, Lawrence B., Eugenin, Jaime, "Optical analysis of circuitry for respiratory rhythm in isolated brainstem of foetal mice", 2008-09, https://doi.org/10.1098/rstb.2009.0070, https://hdl.handle.net/1912/3843
     
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