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dc.contributor.authorAlliegro, Mary Anne  Concept link
dc.contributor.authorHenry, Jonathan J.  Concept link
dc.contributor.authorAlliegro, Mark C.  Concept link
dc.date.accessioned2010-08-04T19:02:56Z
dc.date.available2010-08-04T19:02:56Z
dc.date.issued2010-06
dc.identifier.urihttps://hdl.handle.net/1912/3841
dc.descriptionAuthor Posting. © The Authors, 2010. This is the author's version of the work. It is posted here by permission of National Academy of Sciences for personal use, not for redistribution. The definitive version was published in Proceedings of the National Academy of Sciences of the United States of America 107 (2010): 13718-13723, doi:10.1073/pnas.1008469107.en_US
dc.description.abstractThe nucleolinus is an RNA-rich compartment, closely apposed to or embedded within the nucleolus. Discovered over 150 years ago, fewer than two dozen articles have been published on the nucleolinus, probably because complex histochemical stains are required for its visualization in the great majority of cells. The nucleolinus has been reported in invertebrate oocytes, mammalian and amphibian epithelial cells, neurons, and several transformed cell lines. A prominent nucleolinus, clearly visible with transmitted light microscopes at 10x magnification, is present in each oocyte of the surf clam, Spisula solidissima. We observed a consistent relationship between the nucleolinus and the developing meiotic apparatus following Spisula oocyte activation. Through sonication and sucrose gradient fractionation of purified oocyte nuclei, we isolated nucleolini, extracted their RNA, and prepared an in situ riboprobe (NLi-1) that is associated specifically with the nucleolinus, confirming its unique composition. Other in situ observations revealed a NLi-1 and nucleolinar association with the developing spindle and centrosomes. Laser microsurgery that targeted the nucleolinus resulted in failed meiotic cell division in parthenogenetically activated oocytes and failed mitosis in fertilized oocytes. Although the nucleolinus may be a forgotten organelle, its demonstrated role in spindle formation suggests it deserves renewed attention.en_US
dc.description.sponsorshipThis work was supported by grants from the NIH (GM088503) and NSF (MCB0843092) to MCA. J.H. acknowledges support from the National Science Foundation (IOB 05-16799) and the Directors of the MBL Embryology Courseen_US
dc.format.mimetypeapplication/pdf
dc.language.isoen_USen_US
dc.relation.urihttps://doi.org/10.1073/pnas.1008469107
dc.titleRediscovery of the nucleolinus, a dynamic RNA-rich organelle associated with the nucleolus, spindle, and centrosomesen_US
dc.typePreprinten_US


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