dc.contributor.author | Alliegro, Mary Anne | | |
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dc.contributor.author | Henry, Jonathan J. | | |
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dc.contributor.author | Alliegro, Mark C. | | |
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dc.date.accessioned | 2010-08-04T19:02:56Z | | | |
dc.date.available | 2010-08-04T19:02:56Z | | | |
dc.date.issued | 2010-06 | | | |
dc.identifier.uri | https://hdl.handle.net/1912/3841 | | | |
dc.description | Author Posting. © The Authors, 2010. This is the author's version of the work. It is posted here by permission of National Academy of Sciences for personal use, not for redistribution. The definitive version was published in Proceedings of the National Academy of Sciences of the United States of America 107 (2010): 13718-13723, doi:10.1073/pnas.1008469107. | en_US | | |
dc.description.abstract | The nucleolinus is an RNA-rich compartment, closely apposed to or embedded within
the nucleolus. Discovered over 150 years ago, fewer than two dozen articles have been
published on the nucleolinus, probably because complex histochemical stains are required
for its visualization in the great majority of cells. The nucleolinus has been reported in
invertebrate oocytes, mammalian and amphibian epithelial cells, neurons, and several
transformed cell lines. A prominent nucleolinus, clearly visible with transmitted light
microscopes at 10x magnification, is present in each oocyte of the surf clam, Spisula
solidissima. We observed a consistent relationship between the nucleolinus and the
developing meiotic apparatus following Spisula oocyte activation. Through sonication
and sucrose gradient fractionation of purified oocyte nuclei, we isolated nucleolini,
extracted their RNA, and prepared an in situ riboprobe (NLi-1) that is associated
specifically with the nucleolinus, confirming its unique composition. Other in situ
observations revealed a NLi-1 and nucleolinar association with the developing spindle and
centrosomes. Laser microsurgery that targeted the nucleolinus resulted in failed meiotic
cell division in parthenogenetically activated oocytes and failed mitosis in fertilized
oocytes. Although the nucleolinus may be a forgotten organelle, its demonstrated role in
spindle formation suggests it deserves renewed attention. | en_US | | |
dc.description.sponsorship | This work was supported by grants from
the NIH (GM088503) and NSF (MCB0843092) to MCA. J.H. acknowledges support
from the National Science Foundation (IOB 05-16799) and the Directors of the MBL
Embryology Course | en_US | | |
dc.format.mimetype | application/pdf | | | |
dc.language.iso | en_US | en_US | | |
dc.relation.uri | https://doi.org/10.1073/pnas.1008469107 | | | |
dc.title | Rediscovery of the nucleolinus, a dynamic RNA-rich organelle associated with the nucleolus, spindle, and centrosomes | en_US | | |
dc.type | Preprint | en_US | | |