Windows to cell function and dysfunction : signatures written in the boundary layers
MetadataShow full item record
The medium surrounding cells either in culture or in tissues contains a chemical mix varying with cell state. As solutes move in and out of the cytoplasmic compartment they set up characteristic signatures in the cellular boundary layers. These layers are complex physical and chemical environments whose profiles both reflect cell physiology and provide conduits for intercellular messaging. Here we review some of the most relevant characteristics of the extracellular/intercellular space. Our initial focus is primarily with cultured cells but we extend our consideration to the far more complex environment of tissues and discuss how chemical signatures in the boundary layer can or may affect cell function. Critical to the entire essay are the methods used, or being developed, to monitor chemical profiles in the boundary layers. We review recent developments in ultramicro electrochemical sensors and tailored optical reporters suitable for the task in hand.
Author Posting. © The Author(s), 2010. This is the author's version of the work. It is posted here by permission of John Wiley & Sons for personal use, not for redistribution. The definitive version was published in BioEssays 32 (2010): 514-523, doi:10.1002/bies.200900173.
Suggested CitationPreprint: Smith, Peter J. S., Collis, Leon P., Messerli, Mark A., "Windows to cell function and dysfunction : signatures written in the boundary layers", 2010-01-26, https://doi.org/10.1002/bies.200900173, https://hdl.handle.net/1912/3576
Showing items related by title, author, creator and subject.
Near-inertial kinetic energy budget of the mixed layer and shear evolution in the transition layer in the Arabian Sea during the monsoons Majumder, Sudip; Tandon, Amit; Rudnick, Daniel L.; Farrar, J. Thomas (John Wiley & Sons, 2015-09-26)We present the horizontal kinetic energy (KE) balance of near-inertial currents in the mixed layer and explain shear evolution in the transition layer using observations from a mooring at 15.26° N in the Arabian Sea during ...
Evolution of a Canada Basin ice-ocean boundary layer and mixed layer across a developing thermodynamically forced marginal ice zone Gallaher, Shawn G.; Stanton, Timothy P.; Shaw, William J.; Cole, Sylvia T.; Toole, John M.; Wilkinson, Jeremy P.; Maksym, Ted; Hwang, Byongjun (John Wiley & Sons, 2016-08-22)A comprehensive set of autonomous, ice-ocean measurements were collected across the Canada Basin to study the summer evolution of the ice-ocean boundary layer (IOBL) and ocean mixed layer (OML). Evaluation of local heat ...
Tochko, John Steven (Massachusetts Institute of Technology and Woods Hole Oceanographic Institution, 1978-02)The design and operation of a unique flow measuring instrument for bottom boundary layer studies in the marine environment is documented. The effectiveness of the instrument in acquiring data with which models of near ...