Alkenone producers inferred from well-preserved 18S rDNA in Greenland lake sediments
D'Andrea, William J.
Martiny, Jennifer B. H.
Laatsch, Abby D.
Amaral-Zettler, Linda A.
Sogin, Mitchell L.
MetadataShow full item record
The 18S ribosomal DNA (rDNA) sequences of haptophyte algae were successfully amplified using the polymerase chain reaction (PCR) from water filtrate, surface sediments, and a late-Holocene sediment sample (∼1000 years old) from a group of lakes in the Søndre Strømfjord region of west Greenland. The DNA of the algal primary producer is extremely well preserved in the laminated lake sediments which have been deposited in cold (1°–2°C), anoxic, and sulphidic bottom water. Phylogenetic analyses of the Greenland haptophyte rDNA sequences suggest that alkenones in the Greenland lake sediments are produced by haptophyte algae of the class Prymnesiophyceae. The 18S rDNA sequences from the Greenland samples cluster within a distinct phylotype, differing from both marine haptophytes and from those reported previously from Ace Lake, Antarctica. The similarity of haptophyte rDNA sequences among all samples in this study suggests a single alkenone-based temperature calibration may be applied to these lakes for at least the past 1000 years. These sedimentary archives hold great promise for high-resolution, alkenone-based paleotemperature reconstruction of southern west Greenland, a region sensitive to atmospheric-oceanic climate phenomena such as the North Atlantic Oscillation (NAO).
Author Posting. © American Geophysical Union, 2006. This article is posted here by permission of American Geophysical Union for personal use, not for redistribution. The definitive version was published in Journal of Geophysical Research 111 (2006): G03013, doi:10.1029/2005JG000121.
Showing items related by title, author, creator and subject.
Ancient DNA derived from alkenone-biosynthesizing haptophytes and other algae in Holocene sediments from the Black Sea Coolen, Marco J. L.; Boere, Arjan C.; Abbas, Ben; Baas, Marianne; Wakeham, Stuart G.; Sinninghe Damste, Jaap S. (American Geophysical Union, 2006-02-18)Holocene sea surface temperatures (SST) of the Black Sea have been reconstructed using sedimentary C37 unsaturated alkenones assumed to be derived from the coccolithophorid haptophyte Emiliania huxleyi, whose fossil ...
Global temperature calibration of the alkenone unsaturation index (UK′37) in surface waters and comparison with surface sediments Conte, Maureen H.; Sicre, Marie-Alexandrine; Ruhlemann, Carsten; Weber, John C.; Schulte, Sonja; Schulz-Bull, Detlef; Blanz, Thomas (American Geophysical Union, 2006-02-07)In this paper, we compile the current surface seawater C37 alkenone unsaturation (UK′37) measurements (n=629, −1 to 30°C temperature range) to derive a global, field-based calibration of UK′37 with alkenone production ...
A radiocarbon-based assessment of the preservation characteristics of crenarchaeol and alkenones from continental margin sediments Mollenhauer, Gesine; Eglinton, Timothy I.; Hopmans, Ellen C.; Sinninghe Damste, Jaap S. (2008-01-22)Crenarchaeotal glycerol dibiphytanyl glycerol tetraether (GDGT) lipids and alkenones are two types of biomarkers derived from planktonic marine micro-organisms which are used for reconstruction of sea-surface temperatures. ...