Ancient DNA derived from alkenone-biosynthesizing haptophytes and other algae in Holocene sediments from the Black Sea
Coolen, Marco J. L.
Boere, Arjan C.
Wakeham, Stuart G.
Sinninghe Damste, Jaap S.
MetadataShow full item record
Holocene sea surface temperatures (SST) of the Black Sea have been reconstructed using sedimentary C37 unsaturated alkenones assumed to be derived from the coccolithophorid haptophyte Emiliania huxleyi, whose fossil coccoliths are an important constituent of the unit I sediments. However, alkenones can also be biosynthesized by haptophyte species that do not produce microscopic recognizable coccoliths. A species-specific identification of haptophytes is important in such U 37 K′-based past SST reconstructions since different species have different alkenone-SST calibrations. We showed that 18S rDNA of E. huxleyi made up only a very small percentage (less than 0.8%) of the total eukaryotic 18S rDNA within the up to 3600-year-old fossil record obtained from the depocenter (>2000 m) of the Black Sea. The predominant fossil 18S rDNA was derived from dinoflagellates (Gymnodinium spp.), which are predominant members of the summer phytoplankton bloom in the modern Black Sea. Using a polymerase chain reaction/denaturing gradient gel electrophoresis method selective for haptophytes, we recovered substantial numbers of a preserved 458-base-pair (bp)-long 18S rDNA fragment of E. huxleyi from the Holocene Black Sea sediments. Additional fossil haptophyte sequences were not detected, indicating that the E. huxleyi alkenone-SST calibration can be applied for at least the last ∼3600 years. The ancient E. huxleyi DNA was well protected against degradation since the DNA/alkenone ratio did not significantly decrease throughout the whole sediment core and 20% of ∼2700-year-old fossil E. huxleyi DNA was still up to 23,000 base pairs long. We showed that fossil DNA offers great potential to study the Holocene paleoecology and paleoenvironment of anoxic deep-sea settings in unprecedented detail.
Author Posting. © American Geophysical Union, 2006. This article is posted here by permission of American Geophysical Union for personal use, not for redistribution. The definitive version was published in Paleoceanography 21 (2006): PA1005, doi:10.1029/2005PA001188.
Suggested CitationArticle: Coolen, Marco J. L., Boere, Arjan C., Abbas, Ben, Baas, Marianne, Wakeham, Stuart G., Sinninghe Damste, Jaap S., "Ancient DNA derived from alkenone-biosynthesizing haptophytes and other algae in Holocene sediments from the Black Sea", Paleoceanography 21 (2006): PA1005, DOI:10.1029/2005PA001188, https://hdl.handle.net/1912/3441
Showing items related by title, author, creator and subject.
Arienzo, Monica; McConnell, Joseph R.; Murphy, Lisa N.; Chellman, Nathan; Das, Sarah B.; Kipfstuhl, Sepp; Mulvaney, Robert (John Wiley & Sons, 2017-07-01)Black carbon (BC) and other biomass-burning (BB) aerosols are critical components of climate forcing, but quantification, predictive climate modeling, and policy decisions have been hampered by limited understanding of the ...
Identification of photosynthetic plankton communities using sedimentary ancient DNA and their response to late-Holocene climate change on the Tibetan Plateau Hou, Weiguo; Dong, Hailiang; Li, Gaoyuan; Yang, Jian; Coolen, Marco J. L.; Liu, Xingqi; Wang, Shang; Jiang, Hongchen; Wu, Xia; Xiao, Haiyi; Lian, Bin; Wan, Yunyang (Nature Publishing Group, 2014-10-17)Sediments from Tibetan lakes in NW China are potentially sensitive recorders of climate change and its impact on ecosystem function. However, the important plankton members in many Tibetan Lakes do not make and leave ...
Abrupt changes of temperature and water chemistry in the late Pleistocene and early Holocene Black Sea Bahr, Andre; Lamy, Frank; Arz, Helge W.; Major, Candace O.; Kwiecien, Olga; Wefer, Gerold (American Geophysical Union, 2008-01-12)New Mg/Ca, Sr/Ca, and published stable oxygen isotope and 87Sr/86Sr data obtained on ostracods from gravity cores located on the northwestern Black Sea slope were used to infer changes in the Black Sea hydrology and water ...