Phosphorus physiology of the marine cyanobacterium Trichodesmium
Orchard, Elizabeth Duncan
MetadataShow full item record
KeywordPrimary productivity; Cyanobacteria; Atlantic Exploer (Ship) Cruise; Kilo Moana (Ship) Cruise
Primary producers play a critical role in the oceanic food chain and the global cycling of carbon. The marine diazotroph Trichodesmium is a major contributor to both primary production and nitrogen fixation in the tropical and subtropical oceans. These regions are often characterized by low phosphorus (P) concentrations, and P deficiency of Trichodesmium could limit growth, and constrain nitrogen fixation by this genus. Three key components of P metabolism in cyanobacteria include: inorganic phosphate (Pi) uptake, dissolved organic phosphorus (DOP) hydrolysis, and polyphosphate (polyP) biosynthesis and catabolism. These three pathways lay the foundation for the four chapters that make up the body of this thesis. Chapter two examines the relative importance of Pi and DOP in Trichodesmium in the Sargasso Sea by measuring uptake rates and uptake kinetics of 33Pi and DOP (using alpha labeled adenosine-5’-triphosphate 33P-ATP as a model P ester compound). The in situ uptake rates suggest that the contribution of P ester to total P uptake can be greater than 25% for Trichodesmium, and therefore P esters are important for supplementing Trichodesmium’s P demand. Chapter three confirms the presence of polyP in the Sargasso Sea populations. This is the first detection of polyP in an oligotrophic system and may indicate micro-scale variability in DIP or DOP supply. Chapter four examines the regulation of several genes involved in P scavenging in cultures of Trichodesmium erythraeum IMS101: two copies of a high affinity phosphate binding protein (pstS and sphX) and two putative alkaline phosphatases (phoA and phoX). Chapter five calibrates the expression of phoX to decreases in nitrogen fixation rate in Trichodesmium cultures allowing the use of phoX expression as a molecular indicator of P limitation of nitrogen fixation. Detection of phoX expression in the North Atlantic and South Pacific identified the extent and magnitude of P limitation in these regions. Taken together this thesis provides new insights into how Trichodesmium survives in low P environments, utilizes DOP, stores polyP, and the extent to which P can limit nitrogen fixation by this genus.
Submitted in partial fulfillment of the requirements for the degree of Doctor of Philosophy at the Massachusetts Institute of Technology and the Woods Hole Oceanographic Institution February 2010
Suggested CitationThesis: Orchard, Elizabeth Duncan, "Phosphorus physiology of the marine cyanobacterium Trichodesmium", 2010-02, DOI:10.1575/1912/3366, https://hdl.handle.net/1912/3366
Showing items related by title, author, creator and subject.
The ecology of colonial radiolarians : their colony morphology, trophic interactions and associations, behavior, distribution, and the photosynthesis of their symbionts Swanberg, Neil Ralph (Massachusetts Institute of Technology and Woods Hole Oceanographic Institution, 1979-08)Colonial radiolarians (Spumellaria) are among the most common and abundant large zooplankton, but they have been little studied by modern biologists. Colonies were found on 98% of epipelagic diving stations in the period ...
A determination of air-sea gas exchange and upper ocean biological production from five noble gases and tritiugenic helium-3 Stanley, Rachel H. R. (Massachusetts Institute of Technology and Woods Hole Oceanographic Institution, 2007-09)The five noble gases (helium, neon, argon, krypton, and xenon) are biologically and chemically inert, making them ideal oceanographic tracers. Additionally, the noble gases have a wide range of solubilities and molecular ...
Elkins, Lynne J. (Massachusetts Institute of Technology and Woods Hole Oceanographic Institution, 2009-02)To explore the ability of melting mafic lithologies to produce alkaline ocean-island basalts (OIB), an experimental study was carried out measuring clinopyroxene (Cpx)- melt and garnet (Gt)-melt partition coefficients ...