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    A novel 65 kDa RNA-binding protein in squid presynaptic terminals

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    Author's draft including supplementary figures (1.659Mb)
    Date
    2009-11-09
    Author
    Lico, Diego T. P.  Concept link
    Rosa, José C.  Concept link
    DeGiorgis, Joseph A.  Concept link
    de Vasconcelos, E. J. R.  Concept link
    Casaletti, L.  Concept link
    Tauhata, S. B. F.  Concept link
    Baqui, M. M. A.  Concept link
    Fukuda, M.  Concept link
    Moreira, J. E.  Concept link
    Larson, Roy E.  Concept link
    Metadata
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    Citable URI
    https://hdl.handle.net/1912/3193
    As published
    https://doi.org/10.1016/j.neuroscience.2009.12.005
    Keyword
     Myosin V; hnRNP complex; RNP motifs; Synaptosome; Optic lobe; Loligo 
    Abstract
    A polyclonal antibody (C4), raised against the head domain of chicken myosin Va, reacted strongly towards a 65 kDa polypeptide (p65) on western blots of extracts from squid optic lobes but did not recognize the heavy chain of squid myosin V. This peptide was not recognized by other myosin Va antibodies, nor by an antibody specific for squid myosin V. In an attempt to identify it, p65 was purified from optic lobes of Loligo plei by cationic exchange and reverse phase chromatography. Several peptide sequences were obtained by mass spectroscopy from p65 cut from SDS-PAGE gels. BLAST analysis and partial matching with ESTs from a Loligo pealei data bank indicated that p65 contains consensus signatures for the hnRNP A/B family of RNA-binding proteins. Centrifugation of post mitochondrial extracts from optic lobes on sucrose gradients after treatment with RNase gave biochemical evidence that p65 associates with cytoplasmic RNP complexes in an RNA-dependent manner. Immunohistochemistry and immunofluorescence studies using the C4 antibody showed partial co-labeling with an antibody against squid synaptotagmin in bands within the outer plexiform layer of the optic lobes and at the presynaptic zone of the stellate ganglion. Also, punctate labeling by the C4 antibody was observed within isolated optic lobe synaptosomes. The data indicate that p65 is a novel RNA-binding protein located to the presynaptic terminal within squid neurons and may have a role in synaptic localization of RNA and its translation or processing.
    Description
    Author Posting. © The Author(s), 2009. This is the author's version of the work. It is posted here by permission of Elsevier B.V. for personal use, not for redistribution. The definitive version was published in Neuroscience 166 (2010): 73-83, doi:10.1016/j.neuroscience.2009.12.005.
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    Suggested Citation
    Preprint: Lico, Diego T. P., Rosa, José C., DeGiorgis, Joseph A., de Vasconcelos, E. J. R., Casaletti, L., Tauhata, S. B. F., Baqui, M. M. A., Fukuda, M., Moreira, J. E., Larson, Roy E., "A novel 65 kDa RNA-binding protein in squid presynaptic terminals", 2009-11-09, https://doi.org/10.1016/j.neuroscience.2009.12.005, https://hdl.handle.net/1912/3193
     
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