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dc.contributor.authorVale, Ronald D.
dc.contributor.authorSpudich, James A.
dc.contributor.authorGriffis, Eric R.
dc.date.accessioned2010-03-02T14:48:37Z
dc.date.available2010-03-02T14:48:37Z
dc.date.issued2009-08
dc.identifier.citationJournal of Cell Biology 186 (2009): 727-738en_US
dc.identifier.urihttp://hdl.handle.net/1912/3186
dc.description© The Authors, 2009 . This article is distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported License. The definitive version was published in Journal of Cell Biology 186 (2009): 727-738, doi:10.1083/jcb.200902083.en_US
dc.description.abstractSignals from the mitotic spindle during anaphase specify the location of the actomyosin contractile ring during cytokinesis, but the detailed mechanism remains unresolved. Here, we have imaged the dynamics of green fluorescent protein–tagged myosin filaments, microtubules, and Kinesin-6 (which carries activators of Rho guanosine triphosphatase) at the cell cortex using total internal reflection fluorescence microscopy in flattened Drosophila S2 cells. At anaphase onset, Kinesin-6 relocalizes to microtubule plus ends that grow toward the cortex, but refines its localization over time so that it concentrates on a subset of stable microtubules and along a diffuse cortical band at the equator. The pattern of Kinesin-6 localization closely resembles where new myosin filaments appear at the cortex by de novo assembly. While accumulating at the equator, myosin filaments disappear from the poles of the cell, a process that also requires Kinesin-6 as well as possibly other signals that emanate from the elongating spindle. These results suggest models for how Kinesin-6 might define the position of cortical myosin during cytokinesis.en_US
dc.description.sponsorshipThis work was supported by a National Institutes of Health grant NIH 38499 to R.D. Vale.en_US
dc.format.mimetypeapplication/pdf
dc.language.isoen_USen_US
dc.publisherRockefeller University Pressen_US
dc.relation.urihttp://dx.doi.org/10.1083/jcb.200902083
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/3.0/us/*
dc.titleDynamics of myosin, microtubules, and Kinesin-6 at the cortex during cytokinesis in Drosophila S2 cellsen_US
dc.typeArticleen_US
dc.identifier.doi10.1083/jcb.200902083


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