Prokaryotic community structure in algal photosynthetic biofilms from extreme acidic streams in Rio Tinto (Huelva, Spain)


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dc.contributor.author Souza-Egipsy, Virginia
dc.contributor.author Gonzalez-Toril, Elena
dc.contributor.author Zettler, Erik
dc.contributor.author Amaral-Zettler, Linda A.
dc.contributor.author Aguilera, Angeles
dc.contributor.author Amils, Ricardo
dc.date.accessioned 2010-01-26T15:50:04Z
dc.date.available 2010-01-26T15:50:04Z
dc.date.issued 2008-12
dc.identifier.citation International Microbiology 11 (2008): 251-260 en_US
dc.identifier.uri http://hdl.handle.net/1912/3140
dc.description Author Posting. © Spanish Society for Microbiology (SEM), 2008. This article is posted here by permission of Spanish Society for Microbiology (SEM) for personal use, not for redistribution. The definitive version was published in International Microbiology 11 (2008): 251-260, doi:10.2436/20.1501.01.69. en_US
dc.description.abstract Four algal photosynthetic biofilms were collected from the Rio Tinto (SW Spain) at four localities: AG, Euglena and Pinnularia biofilms; ANG, Chlorella and Pinnularia biofilms; RI, Cyanidium and Dunaliella biofilms; and CEM, Cyanidium, Euglena and Pinnularia biofilms. Community composition and structure were studied by a polyphasic approach consisting of 16S rRNA analysis, scanning electron microscopy by back-scattered electron detection mode (SEM-BSE), and fluorescence in-situ hybridization (FISH). Acidophilic prokaryotes associated with algal photosynthetic biofilms included sequences related to the Alpha-, Beta-, and Gammaproteobacteria (phylum Proteobacteria) and to the phyla Nitrospira, Actinobacteria, Acidobacteria and Firmicutes. Sequences from the Archaea domain were also identified. No more than seven distinct lineages were detected in any biofilm, except for those from RI, which contained fewer groups of Bacteria. Prokaryotic communities of the thinnest algal photosynthetic biofilms (<100 μm) were more related to those in the water column, including Leptospirillum populations. In general, thick biofilms (>200 μm) generate microniches that could facilitate the development of less-adapted microorganisms (coming from the surrounding environment) to extreme conditions, thus resulting in a more diverse prokaryotic biofilm. en_US
dc.description.sponsorship V.SE. and A.A are supported by a Ramón y Cajal contract from the Ministerio de Educación y Ciencia. EG-T. is supported by Instituto Nacional de Técnica Aeroespacial (INTA). This work was supported by a grant from the MECSpain (CGL2005-05470/BOS) and by Centro de Astrobiología (CSICINTA) and a grant from NASA Astrobiology Institute (NC-1054 LAZ). en_US
dc.format.mimetype application/pdf
dc.language.iso en en_US
dc.publisher Spanish Society for Microbiology (SEM) en_US
dc.relation.uri http://dx.doi.org/10.2436/20.1501.01.69
dc.subject Algal biofilms en_US
dc.subject Prokaryotic community en_US
dc.subject Rio Tinto en_US
dc.subject SEM-BSE en_US
dc.subject FISH en_US
dc.subject 16S rRNA en_US
dc.title Prokaryotic community structure in algal photosynthetic biofilms from extreme acidic streams in Rio Tinto (Huelva, Spain) en_US
dc.type Article en_US
dc.identifier.doi 10.2436/20.1501.01.69

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