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    Prokaryotic community structure in algal photosynthetic biofilms from extreme acidic streams in Rio Tinto (Huelva, Spain)

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    IM1104_0251.pdf (474.4Kb)
    Date
    2008-12
    Author
    Souza-Egipsy, Virginia  Concept link
    Gonzalez-Toril, Elena  Concept link
    Zettler, Erik R.  Concept link
    Amaral-Zettler, Linda A.  Concept link
    Aguilera, Angeles  Concept link
    Amils, Ricardo  Concept link
    Metadata
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    Citable URI
    https://hdl.handle.net/1912/3140
    As published
    https://doi.org/10.2436/20.1501.01.69
    DOI
    10.2436/20.1501.01.69
    Keyword
     Algal biofilms; Prokaryotic community; Rio Tinto; SEM-BSE; FISH; 16S rRNA 
    Abstract
    Four algal photosynthetic biofilms were collected from the Rio Tinto (SW Spain) at four localities: AG, Euglena and Pinnularia biofilms; ANG, Chlorella and Pinnularia biofilms; RI, Cyanidium and Dunaliella biofilms; and CEM, Cyanidium, Euglena and Pinnularia biofilms. Community composition and structure were studied by a polyphasic approach consisting of 16S rRNA analysis, scanning electron microscopy by back-scattered electron detection mode (SEM-BSE), and fluorescence in-situ hybridization (FISH). Acidophilic prokaryotes associated with algal photosynthetic biofilms included sequences related to the Alpha-, Beta-, and Gammaproteobacteria (phylum Proteobacteria) and to the phyla Nitrospira, Actinobacteria, Acidobacteria and Firmicutes. Sequences from the Archaea domain were also identified. No more than seven distinct lineages were detected in any biofilm, except for those from RI, which contained fewer groups of Bacteria. Prokaryotic communities of the thinnest algal photosynthetic biofilms (<100 μm) were more related to those in the water column, including Leptospirillum populations. In general, thick biofilms (>200 μm) generate microniches that could facilitate the development of less-adapted microorganisms (coming from the surrounding environment) to extreme conditions, thus resulting in a more diverse prokaryotic biofilm.
    Description
    Author Posting. © Spanish Society for Microbiology (SEM), 2008. This article is posted here by permission of Spanish Society for Microbiology (SEM) for personal use, not for redistribution. The definitive version was published in International Microbiology 11 (2008): 251-260, doi:10.2436/20.1501.01.69.
    Collections
    • Josephine Bay Paul Center in Comparative Molecular Biology and Evolution
    • Faculty Publications
    Suggested Citation
    Article: Souza-Egipsy, Virginia, Gonzalez-Toril, Elena, Zettler, Erik R., Amaral-Zettler, Linda A., Aguilera, Angeles, Amils, Ricardo, "Prokaryotic community structure in algal photosynthetic biofilms from extreme acidic streams in Rio Tinto (Huelva, Spain)", International Microbiology 11 (2008): 251-260, DOI:10.2436/20.1501.01.69, https://hdl.handle.net/1912/3140
     

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