Prokaryotic community structure in algal photosynthetic biofilms from extreme acidic streams in Rio Tinto (Huelva, Spain)
Zettler, Erik R.
Amaral-Zettler, Linda A.
MetadataShow full item record
Four algal photosynthetic biofilms were collected from the Rio Tinto (SW Spain) at four localities: AG, Euglena and Pinnularia biofilms; ANG, Chlorella and Pinnularia biofilms; RI, Cyanidium and Dunaliella biofilms; and CEM, Cyanidium, Euglena and Pinnularia biofilms. Community composition and structure were studied by a polyphasic approach consisting of 16S rRNA analysis, scanning electron microscopy by back-scattered electron detection mode (SEM-BSE), and fluorescence in-situ hybridization (FISH). Acidophilic prokaryotes associated with algal photosynthetic biofilms included sequences related to the Alpha-, Beta-, and Gammaproteobacteria (phylum Proteobacteria) and to the phyla Nitrospira, Actinobacteria, Acidobacteria and Firmicutes. Sequences from the Archaea domain were also identified. No more than seven distinct lineages were detected in any biofilm, except for those from RI, which contained fewer groups of Bacteria. Prokaryotic communities of the thinnest algal photosynthetic biofilms (<100 μm) were more related to those in the water column, including Leptospirillum populations. In general, thick biofilms (>200 μm) generate microniches that could facilitate the development of less-adapted microorganisms (coming from the surrounding environment) to extreme conditions, thus resulting in a more diverse prokaryotic biofilm.
Author Posting. © Spanish Society for Microbiology (SEM), 2008. This article is posted here by permission of Spanish Society for Microbiology (SEM) for personal use, not for redistribution. The definitive version was published in International Microbiology 11 (2008): 251-260, doi:10.2436/20.1501.01.69.
Showing items related by title, author, creator and subject.
Inter-comparison of the potentially active prokaryotic communities in the halocline sediments of Mediterranean deep-sea hypersaline basins Kormas, Konstantinos Ar.; Pachiadaki, Maria G.; Karayanni, Hera; Leadbetter, Edward R.; Bernhard, Joan M.; Edgcomb, Virginia P. (2015-07)The sediment microbiota of the Mediterranean deep-sea anoxic hypersaline basins (DHABs) are understudied relative to communities in the brines and halocline waters. In this study, the active fraction of the prokaryotic ...
Rocke, Emma; Pachiadaki, Maria G.; Cobban, Alec; Kujawinski, Elizabeth B.; Edgcomb, Virginia P. (Public Library of Science, 2015-04-20)Oceanic protist grazing at mesopelagic and bathypelagic depths, and their subsequent effects on trophic links between eukaryotes and prokaryotes, are not well constrained. Recent studies show evidence of higher than expected ...
Distribution and seasonal variability in the benthic eukaryotic community of Río Tinto (SW, Spain), an acidic, high metal extreme environment Aguilera, Angeles; Zettler, Erik R.; Gomez, Felipe; Amaral-Zettler, Linda A.; Rodríguez, Nuria; Amils, Ricardo (2007-02-14)The eukaryotic community of Río Tinto (SW, Spain) was surveyed fall, winter, and spring through the combined use of traditional microscopy and molecular approaches including Denaturing Gradient Gel Electrophoresis (DGGE) ...