Prokaryotic community structure in algal photosynthetic biofilms from extreme acidic streams in Rio Tinto (Huelva, Spain)

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2008-12Author
Souza-Egipsy, Virginia
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Gonzalez-Toril, Elena
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Zettler, Erik R.
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Amaral-Zettler, Linda A.
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Aguilera, Angeles
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Amils, Ricardo
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https://hdl.handle.net/1912/3140As published
https://doi.org/10.2436/20.1501.01.69DOI
10.2436/20.1501.01.69Keyword
Algal biofilms; Prokaryotic community; Rio Tinto; SEM-BSE; FISH; 16S rRNAAbstract
Four algal photosynthetic biofilms were collected from the Rio Tinto (SW Spain) at four localities: AG, Euglena and
Pinnularia biofilms; ANG, Chlorella and Pinnularia biofilms; RI, Cyanidium and Dunaliella biofilms; and CEM, Cyanidium,
Euglena and Pinnularia biofilms. Community composition and structure were studied by a polyphasic approach consisting of 16S
rRNA analysis, scanning electron microscopy by back-scattered electron detection mode (SEM-BSE), and fluorescence in-situ
hybridization (FISH). Acidophilic prokaryotes associated with algal photosynthetic biofilms included sequences related to the
Alpha-, Beta-, and Gammaproteobacteria (phylum Proteobacteria) and to the phyla Nitrospira, Actinobacteria, Acidobacteria and
Firmicutes. Sequences from the Archaea domain were also identified. No more than seven distinct lineages were detected in any
biofilm, except for those from RI, which contained fewer groups of Bacteria. Prokaryotic communities of the thinnest algal photosynthetic
biofilms (<100 μm) were more related to those in the water column, including Leptospirillum populations. In general, thick
biofilms (>200 μm) generate microniches that could facilitate the development of less-adapted microorganisms (coming from the
surrounding environment) to extreme conditions, thus resulting in a more diverse prokaryotic biofilm.
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Author Posting. © Spanish Society for Microbiology (SEM), 2008. This article is posted here by permission of Spanish Society for Microbiology (SEM) for personal use, not for redistribution. The definitive version was published in International Microbiology 11 (2008): 251-260, doi:10.2436/20.1501.01.69.
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