Protistan community patterns within the brine and halocline of deep hypersaline anoxic basins in the eastern Mediterranean Sea
Edgcomb, Virginia P.
Orsi, William D.
Epstein, Slava S.
Yakimov, Michail M.
MetadataShow full item record
KeywordAnoxic; Brine; Community structure; Deep-sea; DHAB; Hypersaline; Molecular diversity; Protists
Environmental factors restrict the distribution of microbial eukaryotes but the exact boundaries for eukaryotic life are not known. Here we examine protistan communities at the extremes of salinity and osmotic pressure, and report rich assemblages inhabiting Bannock and Discovery, two deep-sea superhaline anoxic basins in the Mediterranean. Using a rRNA-based approach, we detected 1538 protistan rRNA gene sequences from water samples with total salinity ranging from 39 g/kg to 280 g/Kg, and obtained evidence that this DNA was endogenous to the extreme habitats sampled. Statistical analyses indicate that the discovered phylotypes represent only a fraction of species actually inhabiting both the brine and the brine-seawater interface, with as much as 82% of the actual richness missed by our survey. Jaccard indices (e.g., for a comparison of community membership) suggest that the brine/interface protistan communities are unique to Bannock and Discovery basins, and share little (0.8-2.8%) in species composition with overlying waters with typical marine salinity and oxygen tension. The protistan communities from the basins’ brine and brine/seawater interface appear to be particularly enriched with dinoflagellates, ciliates and other alveolates, as well as fungi, and are conspicuously poor in stramenopiles. The uniqueness and diversity of brine and brine-interface protistan communities make them promising targets for protistan discovery.
Author Posting. © The Author(s), 2008. This is the author's version of the work. It is posted here by permission of Springer for personal use, not for redistribution. The definitive version was published in Extremophiles 13 (2009): 151-167, doi:10.1007/s00792-008-0206-2.
Suggested CitationPreprint: Edgcomb, Virginia P., Orsi, William D., Leslin, Chesley, Epstein, Slava S., Bunge, John, Jeon, Sunok, Yakimov, Michail M., Behnke, Anke, Stoeck, Thorsten, "Protistan community patterns within the brine and halocline of deep hypersaline anoxic basins in the eastern Mediterranean Sea", 2008-10-21, https://doi.org/10.1007/s00792-008-0206-2, https://hdl.handle.net/1912/3084
Showing items related by title, author, creator and subject.
Inter-comparison of the potentially active prokaryotic communities in the halocline sediments of Mediterranean deep-sea hypersaline basins Kormas, Konstantinos Ar.; Pachiadaki, Maria G.; Karayanni, Hera; Leadbetter, Edward R.; Bernhard, Joan M.; Edgcomb, Virginia P. (2015-07)The sediment microbiota of the Mediterranean deep-sea anoxic hypersaline basins (DHABs) are understudied relative to communities in the brines and halocline waters. In this study, the active fraction of the prokaryotic ...
Bernhard, Joan M.; Morrison, Colin R.; Pape, Ellen; Beaudoin, David J.; Todaro, M. Antonio; Pachiadaki, Maria G.; Kormas, Konstantinos Ar.; Edgcomb, Virginia P. (BioMed Central, 2015-12-10)The deep-sea hypersaline anoxic basins (DHABs) of the Mediterranean (water depth ~3500 m) are some of the most extreme oceanic habitats known. Brines of DHABs are nearly saturated with salt, leading many to suspect they ...
Supplemental files from “Metazoans of redoxcline sediments in Mediterranean deep-sea hypersaline anoxic basins” (Bernhard et al., submitted, BMC Biology). Bernhard, Joan M.; Morrison, Colin R.; Pape, Ellen; Beaudoin, David J.; Todaro, M. Antonio; Pachiadaki, Maria G.; Kormas, Konstantinos Ar.; Edgcomb, Virginia P. (2015-10-05)Link provides access to supplemental tables and figures to our manuscript regarding metazoans of redoxcline sediments in Mediterranean deep-sea hypersaline anoxic basins (DHABs). Specimens shown in supplemental figures are ...