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    Migration and actin protrusion in melanoma cells are regulated by EB1 protein

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    Schober manuscript complete.pdf (834.8Kb)
    Date
    2009-04
    Author
    Schober, Joseph M.  Concept link
    Cain, Jeanine M.  Concept link
    Komarova, Yulia A.  Concept link
    Borisy, Gary G.  Concept link
    Metadata
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    Citable URI
    https://hdl.handle.net/1912/3048
    As published
    https://doi.org/10.1016/j.canlet.2009.04.007
    Keyword
     Melanoma; EB1; Microtubules; Actin; Motility 
    Abstract
    Remodeling of actin and microtubule cytoskeletons is thought to be coupled; however, the interplay between these two systems is not fully understood. We show a microtubule end-binding protein, EB1, is required for formation of polarized morphology and motility of melanoma cells. EB1 depletion decreased lamellipodia protrusion, and resulted in loss of opposed protruding and retracting cell edges. Lamellipodia attenuation correlated with mis-localization of filopodia throughout the cell and decreased Arp3 localization. EB1-depleted cells displayed less persistent migration and reduced velocity in singlecell motility experiments. We propose EB1 coordinates melanoma cell migration through regulating the balance between lamellipodial and filopodial protrusion.
    Description
    Author Posting. © The Author(s), 2009. This is the author's version of the work. It is posted here by permission of Elsevier B.V. for personal use, not for redistribution. The definitive version was published in Cancer Letters 284 (2009): 30-36, doi:10.1016/j.canlet.2009.04.007.
    Collections
    • Cellular Dynamics Program
    Suggested Citation
    Preprint: Schober, Joseph M., Cain, Jeanine M., Komarova, Yulia A., Borisy, Gary G., "Migration and actin protrusion in melanoma cells are regulated by EB1 protein", 2009-04, https://doi.org/10.1016/j.canlet.2009.04.007, https://hdl.handle.net/1912/3048
     

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