Rotifer rDNA-specific R9 retrotransposable elements generate an exceptionally long target site duplication upon insertion
MetadataShow full item record
Ribosomal DNA genes in many eukaryotes contain insertions of non-LTR retrotransposable elements belonging to the R2 clade. These elements persist in the host genomes by inserting site-specifically into multicopy target sites, thereby avoiding random disruption of single-copy host genes. Here we describe R9 retrotransposons from the R2 clade in the 28S RNA genes of bdelloid rotifers, small freshwater invertebrate animals best known for their long-term asexuality and for their ability to survive repeated cycles of desiccation and rehydration. While the structural organization of R9 elements is highly similar to that of other members of the R2 clade, they are characterized by two distinct features: sitespecific insertion into a previously unreported target sequence within the 28S gene, and an unusually long target site duplication of 126 bp. We discuss the implications of these findings in the context of bdelloid genome organization and the mechanisms of target-primed reverse transcription.
Author Posting. © The Author(s), 2009. This is the author's version of the work. It is posted here by permission of Elsevier B.V. for personal use, not for redistribution. The definitive version was published in Gene 448 (2009): 145-150, doi:10.1016/j.gene.2009.08.016.
Suggested CitationPreprint: Gladyshev, Eugene A., Arkhipova, Irina R., "Rotifer rDNA-specific R9 retrotransposable elements generate an exceptionally long target site duplication upon insertion", 2009-08, https://doi.org/10.1016/j.gene.2009.08.016, https://hdl.handle.net/1912/3006
Showing items related by title, author, creator and subject.
Endonuclease-containing Penelope retrotransposons in the bdelloid rotifer Adineta vaga exhibit unusual structural features and play a role in expansion of host gene families Arkhipova, Irina R.; Yushenova, Irina A.; Rodriguez, Fernando (BioMed Central, 2013-08-27)Penelope-like elements (PLEs) are an enigmatic group of retroelements sharing a common ancestor with telomerase reverse transcriptases. In our previous studies, we identified endonuclease-deficient PLEs that are associated ...
Snell, Terry W.; Johnston, Rachel K.; Gribble, Kristin E.; Mark Welch, David B. (Taylor & Francis, 2014-06-12)Comparative biogerontology has much to contribute to the study of aging. A broad range of aging rates have evolved to meet environmental challenges, and understanding these adaptations can produce valuable insights into ...
LTR-retrotransposons from Bdelloid rotifers capture additional ORFs shared between highly diverse retroelement types Rodriguez, Fernando; Kenefick, Aubrey W.; Arkhipova, Irina R. (MDPI AG, 2017-04-11)Rotifers of the class Bdelloidea, microscopic freshwater invertebrates, possess a highlydiversified repertoire of transposon families, which, however, occupy less than 4% of genomic DNA in the sequenced representative ...