Now showing items 657-676 of 883

    • Quantification of cuttlefish (Sepia officinalis) camouflage : a study of color and luminance using in situ spectrometry 

      Akkaynak, Derya; Allen, Justine J.; Mathger, Lydia M.; Chiao, Chuan-Chin; Hanlon, Roger T. (2012-11-19)
      Cephalopods are renowned for their ability to adaptively camouflage on diverse backgrounds. Sepia officinalis camouflage body patterns have been characterized spectrally in the laboratory but not in the field due to the ...
    • Quantifying the production of dissolved organic nitrogen in headwater streams using 15N tracer additions 

      Johnson, Laura T.; Tank, Jennifer L.; Hall, Robert O.; Mulholland, Patrick J.; Hamilton, Stephen K.; Valett, H. Maurice; Webster, Jackson R.; Bernot, Melody J.; McDowell, William H.; Peterson, Bruce J.; Thomas, Suzanne M. (Association for the Sciences of Limnology and Oceanography, 2013-07)
      Most nitrogen (N) assimilation in lake and marine ecosystems is often subsequently released via autochthonous dissolved organic nitrogen (DON) production, but autochthonous DON production has yet to be quantified in flowing ...
    • Quantitative analysis and modeling of katanin function in flagellar length control 

      Kannegaard, Elisa; Rego, E. Hesper; Schuck, Sebastian; Feldman, Jessica L.; Marshall, Wallace F. (American Society for Cell Biology, 2014-08-20)
      Flagellar length control in Chlamydomonas reinhardtii provides a simple model system in which to investigate the general question of how cells regulate organelle size. Previous work demonstrated that Chlamydomonas cytoplasm ...
    • Quantitative biology : where modern biology meets physical sciences 

      Shekhar, Shashank; Zhu, Lian; Mazutis, Linas; Sgro, Allyson E.; Fai, Thomas G.; Podolski, Marija (American Society for Cell Biology, 2014-11-05)
      Quantitative methods and approaches have been playing an increasingly important role in cell biology in recent years. They involve making accurate measurements to test a predefined hypothesis in order to compare experimental ...
    • Quantitative measurements and modeling of cargo–motor interactions during fast transport in the living axon 

      Seamster, Pamela E.; Loewenberg, Michael; Pascal, Jennifer; Chauviere, Arnaud; Gonzales, Aaron; Cristini, Vittorio; Bearer, Elaine L. (IOP Publishing, 2012-09-25)
      The kinesins have long been known to drive microtubule-based transport of sub-cellular components, yet the mechanisms of their attachment to cargo remain a mystery. Several different cargo-receptors have been proposed based ...
    • Quantitative orientation-independent differential interference contrast (DIC) microscopy coupled with orientation-independent Polarization microscopy 

      Shribak, Michael; LaFountain, James R.; Biggs, David; Inoue, Shinya (Cambridge University Press, 2007-08-05)
      Differential interference contrast (DIC) microscopy is widely used to observe structure and motion in unstained, transparent living cells and isolated organelles, producing a monochromatic shadowcast image of optical ...
    • Quantitative orientation-independent differential interference contrast microscope with fast switching shear direction and bias modulation 

      Shribak, Michael (Optical Society of America, 2013-03-27)
      We describe a quantitative orientation-independent differential interference contrast (DIC) microscope, which allows bias retardation to be modulated and shear directions to be switched rapidly without any mechanical ...
    • A quantitative reference transcriptome for Nematostella vectensis early embryonic development : a pipeline for de novo assembly in emerging model systems 

      Tulin, Sarah; Aguiar, Derek; Istrail, Sorin; Smith, Joel (BioMed Central, 2013-06-03)
      The de novo assembly of transcriptomes from short shotgun sequences raises challenges due to random and non-random sequencing biases and inherent transcript complexity. We sought to define a pipeline for de novo transcriptome ...
    • Rapid recovery of genetic diversity of stomatopod populations on Krakatau : temporal and spatial scales of marine larval dispersal 

      Barber, Paul H.; Moosa, M. K.; Palumbi, S. R. (Royal Society, 2002-07-09)
      Although the recovery of terrestrial communities shattered by the massive eruption of Krakatau in 1883 has been well chronicled, the fate of marine populations has been largely ignored. We examined patterns of genetic ...
    • Rapid screening for freshwater bacterial groups by using reverse line blot hybridization 

      Zwart, Gabriel; van Hannen, Erik J.; Kamst-van Agterveld, Miranda P.; Van der Gucht, Katleen; Lindstrom, Eva S.; Van Wichelen, Jeroen; Lauridsen, Torben; Crump, Byron C.; Han, Suk-Kyun; Declerck, Steven (American Society for Microbiology, 2003-10)
      The identification of phylogenetic clusters of bacteria that are common in freshwater has provided a basis for probe design to target important freshwater groups. We present a set of 16S ribosomal RNA gene-based oligonucleotide ...
    • Rapid, low-input, low-bias construction of shotgun fragment libraries by high-density in vitro transposition 

      Adey, Andrew; Morrison, Hilary G.; Asan; Xun, Xu; Kitzman, Jacob O.; Turner, Emily H.; Stackhouse, Bethany; MacKenzie, Alexandra P.; Caruccio, Nicholas C.; Zhang, Xiuqing; Shendure, Jay (BioMed Central Ltd, 2010-12-08)
      We characterize and extend a highly efficient method for constructing shotgun fragment libraries in which transposase catalyzes in vitro DNA fragmentation and adaptor incorporation simultaneously. We apply this method to ...
    • RCN4GSC Workshop Report : managing data at the interface of biodiversity and (meta)genomics, March 2011 

      Robbins, Robert J.; Amaral-Zettler, Linda A.; Bik, Holly; Blum, Stan D.; Edwards, James; Field, Dawn; Garrity, George M.; Gilbert, Jack A.; Kottmann, Renzo; Krishtalka, Leonard; Lapp, Hilmar; Lawrence, Carolyn; Morrison, Norman; O Tuama, Eamonn; Parr, Cynthia Sims; San Gil, Inigo; Schindel, David; Schriml, Lynn M.; Vieglas, David; Wooley, John (Genomic Standards Consortium, 2012-07-28)
      Building on the planning efforts of the RCN4GSC project, a workshop was convened in San Diego to bring together experts from genomics and metagenomics, biodiversity, ecology, and bioinformatics with the charge to identify ...
    • Recent changes in nitrate and dissolved organic carbon export from the upper Kuparuk River, North Slope, Alaska 

      McClelland, James W.; Stieglitz, Marc; Pan, Feifei; Holmes, Robert M.; Peterson, Bruce J. (American Geophysical Union, 2007-11-08)
      Export of nitrate and dissolved organic carbon (DOC) from the upper Kuparuk River between the late 1970s and early 2000s was evaluated using long-term ecological research (LTER) data in combination with solute flux and ...
    • Reconciling carbon-cycle concepts, terminology, and methods 

      Chapin, F. Stuart; Woodwell, G. M.; Randerson, James T.; Rastetter, Edward B.; Lovett, G. M.; Baldocchi, Dennis D.; Clark, D. A.; Harmon, Mark E.; Schimel, David S.; Valentini, R.; Wirth, C.; Aber, J. D.; Cole, Jonathan J.; Goulden, Michael L.; Harden, J. W.; Heimann, M.; Howarth, Robert W.; Matson, P. A.; McGuire, A. David; Melillo, Jerry M.; Mooney, H. A.; Neff, Jason C.; Houghton, Richard A.; Pace, Michael L.; Ryan, M. G.; Running, Steven W.; Sala, Osvaldo E.; Schlesinger, William H.; Schulze, E.-D. (2006-01-06)
      Recent patterns and projections of climatic change have focused increased scientific and public attention on patterns of carbon (C) cycling and its controls, particularly the factors that determine whether an ecosystem is ...
    • Reconfigurable self-assembly through chiral control of interfacial tension 

      Gibaud, Thomas; Barry, Edward; Zakhary, Mark J.; Henglin, Mir; Ward, Andrew; Yang, Yasheng; Berciu, Cristina; Oldenbourg, Rudolf; Hagan, Michael F.; Nicastro, Daniela; Meyer, Robert B.; Dogic, Zvonimir (2011-12-01)
      From determining optical properties of simple molecular crystals to establishing preferred handedness in highly complex vertebrates, molecular chirality profoundly influences the structural, mechanical, and optical properties ...
    • Reconstitution of amoeboid motility in vitro identifies a motor-independent mechanism for cell body retraction 

      Shimabukuro, Katsuya; Noda, Naoki; Stewart, Murray; Roberts, Thomas M. (2011-08)
      Crawling movement in eukaryotic cells requires coordination of leading edge protrusion with cell body retraction [1-3]. Protrusion is driven by actin polymerization along the leading edge [4]. The mechanism of retraction ...
    • Reconstitution of dynamic microtubules with Drosophila XMAP215, EB1, and Sentin 

      Li, Wenjing; Moriwaki, Takashi; Tani, Tomomi; Watanabe, Takashi; Kaibuchi, Kozo; Goshima, Gohta (Rockefeller University Press, 2012-11-26)
      Dynamic microtubules (MTs) are essential for various intracellular events, such as mitosis. In Drosophila melanogaster S2 cells, three MT tip-localizing proteins, Msps/XMAP215, EB1, and Sentin (an EB1 cargo protein), have ...
    • Reconstructing rare soil microbial genomes using in situ enrichments and metagenomics 

      Delmont, Tom O.; Eren, A. Murat; Maccario, Lorrie; Prestat, Emmanuel; Esen, Ozcan C.; Pelletier, Eric; Le Paslier, Denis; Simonet, Pascal; Vogel, Timothy M. (Frontiers Media, 2015-04-30)
      Despite extensive direct sequencing efforts and advanced analytical tools, reconstructing microbial genomes from soil using metagenomics have been challenging due to the tremendous diversity and relatively uniform distribution ...
    • Recovering complete and draft population genomes from metagenome datasets 

      Sangwan, Naseer; Xia, Fangfang; Gilbert, Jack A. (BioMed Central, 2016-03-08)
      Assembly of metagenomic sequence data into microbial genomes is of fundamental value to improving our understanding of microbial ecology and metabolism by elucidating the functional potential of hard-to-culture microorganisms. ...
    • Recovery from disturbance requires resynchronization of ecosystem nutrient cycles 

      Rastetter, Edward B.; Yanai, Ruth D.; Thomas, R. Q.; Vadeboncoeur, M. A.; Fahey, Timothy J.; Fisk, M. C.; Kwiatkowski, Bonnie L.; Hamburg, S. P. (Ecological Society of America, 2013-04)
      Nitrogen (N) and phosphorus (P) are tightly cycled in most terrestrial ecosystems, with plant uptake more than 10 times higher than the rate of supply from deposition and weathering. This near-total dependence on recycled ...