Now showing items 537-556 of 812

  • Obelin mutants as reporters in bioluminescent dual-analyte binding assay 

    Krasitskaya, Vasilisa V.; Kudryavtsev, Alexander N.; Shimomura, Osamu; Frank, Ludmila A. (RSC Publishing, 2012-11-29)
    Two obelin mutants distinctly different in the color of bioluminescence were successfully applied to simultaneous detection of two analytes in a single well. The flash-type signals were triggered by single injection of ...
  • Objective indicators of pasture degradation from spectral mixture analysis of Landsat imagery 

    Davidson, Eric A.; Asner, Gregory P.; Stone, Thomas A.; Neill, Christopher; Figueiredo, Ricardo O. (American Geophysical Union, 2008-07-23)
    Degradation of cattle pastures is a management concern that influences future land use in Amazonia. However, “degradation” is poorly defined and has different meanings for ranchers, ecologists, and policy makers. Here we ...
  • The ocean sampling day consortium 

    Kopf, Anna; Bicak, Mesude; Kottmann, Renzo; Schnetzer, Julia; Kostadinov, Ivaylo; Lehmann, Katja; Fernandez-Guerra, Antonio; Jeanthon, Christian; Rahav, Eyal; Ullrich, Matthias; Wichels, Antje; Gerdts, Gunnar; Polymenakou, Paraskevi; Kotoulas, Giorgos; Siam, Rania; Abdallah, Rehab Z.; Sonnenschein, Eva C.; Cariou, Thierry; O’Gara, Fergal; Jackson, Stephen; Orlic, Sandi; Steinke, Michael; Busch, Julia; Duarte, Bernardo; Cacador, Isabel; Canning-Clode, Joao; Bobrova, Oleksandra; Marteinsson, Viggo; Reynisson, Eyjolfur; Loureiro, Clara Magalhaes; Luna, Gian Marco; Quero, Grazia Marina; Loscher, Carolin R.; Kremp, Anke; DeLorenzo, Marie E.; Øvreås, Lise; Tolman, Jennifer; LaRoche, Julie; Penna, Antonella; Frischer, Marc; Davis, Timothy; Katherine, Barker; Meyer, Christopher P.; Ramos, Sandra; Magalhaes, Catarina; Jude-Lemeilleur, Florence; Aguirre-Macedo, Ma Leopoldina; Wang, Shiao; Poulton, Nicole; Jones, Scott; Collin, Rachel; Fuhrman, Jed A.; Conan, Pascal; Alonso, Cecilia; Stambler, Noga; Goodwin, Kelly; Yakimov, Michael M.; Baltar, Federico; Bodrossy, Levente; Van De Kamp, Jodie; Frampton, Dion M. F.; Ostrowski, Martin; Van Ruth, Paul; Malthouse, Paul; Claus, Simon; Deneudt, Klaas; Mortelmans, Jonas; Pitois, Sophie; Wallom, David; Salter, Ian; Costa, Rodrigo; Schroeder, Declan C.; Kandil, Mahrous M.; Amaral, Valentina; Biancalana, Florencia; Santana, Rafael; Pedrotti, Maria Luiza; Yoshida, Takashi; Ogata, Hiroyuki; Ingleton, Tim; Munnik, Kate; Rodriguez-Ezpeleta, Naiara; Berteaux-Lecellier, Veronique; Wecker, Patricia; Cancio, Ibon; Vaulot, Daniel; Bienhold, Christina; Ghazal, Hassan; Chaouni, Bouchra; Essayeh, Soumya; Ettamimi, Sara; Zaid, El Houcine; Boukhatem, Noureddine; Bouali, Abderrahim; Chahboune, Rajaa; Barrijal, Said; Timinouni, Mohammed; El Otmani, Fatima; Bennani, Mohamed; Mea, Marianna; Todorova, Nadezhda; Karamfilov, Ventzislav; ten Hoopen, Petra; Cochrane, Guy; L’Haridon, Stephane; Bizsel, Kemal Can; Vezzi, Alessandro; Lauro, Federico M.; Martin, Patrick; Jensen, Rachelle M.; Hinks, Jamie; Gebbels, Susan; Rosselli, Riccardo; De Pascale, Fabio; Schiavon, Riccardo; dos Santos, Antonina; Villar, Emilie; Pesant, Stephane; Cataletto, Bruno; Malfatti, Francesca; Edirisinghe, Ranjith; Herrera Silveira, Jorge A.; Barbier, Michele; Turk, Valentina; Tinta, Tinkara; Fuller, Wayne J.; Salihoglu, Ilkay; Serakinci, Nedime; Ergoren, Mahmut Cerkez; Bresnan, Eileen; Iriberri, Juan; Fronth Nyhus, Paul Anders; Bente, Edvardsen; Karlsen, Hans Erik; Golyshin, Peter N.; Gasol, Josep M.; Moncheva, Snejana; Dzhembekova, Nina; Johnson, Zackary; Sinigalliano, Christopher David; Gidley, Maribeth Louise; Zingone, Adriana; Danovaro, Roberto; Tsiamis, George; Clark, Melody S.; Costa, Ana Cristina; El Bour, Monia; Martins, Ana M.; Collins, R. Eric; Ducluzeau, Anne-Lise; Martinez, Jonathan; Costello, Mark J.; Amaral-Zettler, Linda A.; Gilbert, Jack A.; Davies, Neil; Field, Dawn; Glockner, Frank Oliver (BioMed Central, 2015-06-19)
    Ocean Sampling Day was initiated by the EU-funded Micro B3 (Marine Microbial Biodiversity, Bioinformatics, Biotechnology) project to obtain a snapshot of the marine microbial biodiversity and function of the world’s oceans. ...
  • Oceanic heterotrophic bacterial nutrition by semilabile DOM as revealed by data assimilative modeling 

    Luo, Ya-Wei; Friedrichs, Marjorie A. M.; Doney, Scott C.; Church, Matthew J.; Ducklow, Hugh W. (Inter-Research, 2010-08-03)
    Previous studies have focused on the role of labile dissolved organic matter (DOM) (defined as turnover time of ~1 d) in supporting heterotrophic bacterial production, but have mostly neglected semilabile DOM (defined as ...
  • Odorant specificity of three oscillations and the DC signal in the turtle olfactory bulb 

    Lam, Ying-Wan; Cohen, Lawrence B.; Zochowski, Michal R. (2002-11-16)
    The odour-induced population response in the in vivo turtle (Terepene sp.) olfactory bulb consists of three oscillatory components (rostral, middle and caudal) that ride on top of a DC signal. In an initial step to determine ...
  • Oligotyping : differentiating between closely related microbial taxa using 16S rRNA gene data 

    Eren, A. Murat; Maignien, Lois; Sul, Woo Jun; Murphy, Leslie G.; Grim, Sharon L.; Morrison, Hilary G.; Sogin, Mitchell L. (John Wiley & Sons, 2013-10-23)
    Bacteria comprise the most diverse domain of life on Earth, where they occupy nearly every possible ecological niche and play key roles in biological and chemical processes. Studying the composition and ecology of bacterial ...
  • Oligotyping reveals community level habitat selection within the genus Vibrio 

    Schmidt, Victor T.; Reveillaud, Julie; Zettler, Erik R.; Mincer, Tracy J.; Murphy, Leslie G.; Amaral-Zettler, Linda A. (Frontiers Media, 2014-11-13)
    The genus Vibrio is a metabolically diverse group of facultative anaerobic bacteria, common in aquatic environments and marine hosts. The genus contains several species of importance to human health and aquaculture, including ...
  • On the gating mechanisms of the light-dependent conductance in Pecten hyperpolarizing photoreceptors : does light remove inactivation in voltage-dependent K channels? 

    Gomez, Maria del Pilar; Nasi, Enrico (Rockefeller University Press, 2005-04-11)
    The hyperpolarizing receptor potential of ciliary photoreceptors of scallop and other mollusks is mediated by a cGMP-activated K conductance; these cells also express a transient potassium current triggered by depolarization. ...
  • One nutritional symbiosis begat another : Phylogenetic evidence that the ant tribe Camponotini acquired Blochmannia by tending sap-feeding insects 

    Wernegreen, Jennifer J.; Kauppinen, Seth N.; Brady, Sean G.; Ward, Philip S. (BioMed Central, 2009-12-16)
    Bacterial endosymbiosis has a recurring significance in the evolution of insects. An estimated 10-20% of insect species depend on bacterial associates for their nutrition and reproductive viability. Members of the ant tribe ...
  • Optical analysis of circuitry for respiratory rhythm in isolated brainstem of foetal mice 

    Muller, Kenneth J.; Tsechpenakis, Gavriil; Homma, Ryota; Nicholls, John G.; Cohen, Lawrence B.; Eugenin, Jaime (2008-09)
    Respiratory rhythms arise from neurons situated in the ventral medulla. We are investigating their spatial and functional relationships optically by measuring changes in intracellular calcium using the fluorescent, ...
  • Optical instruments for measuring leaf area index in low vegetation : application in Arctic ecosystems 

    van Wijk, Mark T.; Williams, Mathew (Ecological Society of America, 2005-08)
    Leaf area index (LAI) is a powerful diagnostic of plant productivity. Despite the fact that many methods have been developed to quantify LAI, both directly and indirectly, leaf area index remains difficult to quantify ...
  • Optical map of the genotype A1 WB C6 Giardia lamblia genome isolate 

    Perry, D. Alexander; Morrison, Hilary G.; Adam, Rodney D. (2011-07)
    The Giardia lamblia genome consists of 12 Mb divided among 5 chromosomes ranging in size from approximately 1 to 4 Mb. The assembled contigs of the genotype A1 isolate, WB, were previously mapped along the 5 chromosomes ...
  • Optical recording from respiratory pattern generator of fetal mouse brainstem reveals a distributed network 

    Eugenin, Jaime; Nicholls, John G.; Cohen, Lawrence B.; Muller, Kenneth J. (2005-10-17)
    Unfailing respiration depends on neural mechanisms already present in mammals before birth. Experiments were made to determine how inspiratory and expiratory neurons are grouped in the brainstem of fetal mice. A further ...
  • Optimal stimulus shapes for neuronal excitation 

    Forger, Daniel B.; Paydarfar, David; Clay, John R. (Public Library of Science, 2011-07-07)
    An important problem in neuronal computation is to discern how features of stimuli control the timing of action potentials. One aspect of this problem is to determine how an action potential, or spike, can be elicited with ...
  • Optimization of a GCaMP calcium indicator for neural activity imaging 

    Akerboom, Jasper; Chen, Tsai-Wen; Wardill, Trevor J.; Tian, Lin; Marvin, Jonathan S.; Mutlu, Sevinc; Calderon, Nicole Carreras; Esposti, Federico; Borghuis, Bart G.; Sun, Xiaonan Richard; Gordus, Andrew; Orger, Michael B.; Portugues, Ruben; Engert, Florian; Macklin, John J.; Filosa, Alessandro; Aggarwal, Aman; Kerr, Rex A.; Takagi, Ryousuke; Kracun, Sebastian; Shigetomi, Eiji; Khakh, Baljit S.; Baier, Herwig; Lagnado, Leon; Wang, Samuel S.-H.; Bargmann, Cornelia I.; Kimmel, Bruce E.; Jayaraman, Vivek; Svoboda, Karel; Kim, Douglas S.; Schreiter, Eric R.; Looger, Loren L. (Society for Neuroscience, 2012-10-03)
    Genetically encoded calcium indicators (GECIs) are powerful tools for systems neuroscience. Recent efforts in protein engineering have significantly increased the performance of GECIs. The state-of-the art single-wavelength ...
  • Optimized ratiometric calcium sensors for functional in vivo imaging of neurons and T lymphocytes 

    Thestrup, Thomas; Litzlbauer, Julia; Bartholomaus, Ingo; Mues, Marsilius; Russo, Luigi; Dana, Hod; Kovalchuk, Yuri; Liang, Yajie; Kalamakis, Georgios; Lauka, Yvonne; Becker, Stefan; Witte, Gregor; Geiger, Anselm; Allen, Taylor; Rome, Lawrence C.; Chen, Tsai-Wen; Kim, Douglas S.; Garaschuk, Olga; Griesinger, Christian; Griesbeck, Oliver (2013-06)
    The quality of genetically encoded calcium indicators (GECIs) has improved dramatically in recent years, but high-performing ratiometric indicators are still rare. Here we describe a series of fluorescence resonance energy ...
  • Optogenetic mapping of cerebellar inhibitory circuitry reveals spatially biased coordination of interneurons via electrical synapses 

    Kim, Jinsook; Lee, Soojung; Tsuda, Sachiko; Zhang, Xuying; Asrican, Brent; Gloss, Bernd; Feng, Guoping; Augustine, George J. (Cell Press, 2014-05-22)
    We used high-speed optogenetic mapping technology to examine the spatial organization of local inhibitory circuits formed by cerebellar interneurons. Transgenic mice expressing channelrhodopsin-2 exclusively in molecular ...
  • Oral administration of pharmacologically active substances to squid : a methodological description 

    Berk, William; Teperman, Jake; Walton, Kerry D.; Hirata, Kazunari; Sugimori, Mutsuyuki; Llinas, Rodolfo R. (Marine Biological Laboratory, 2009-02)
    The squid giant synapse is a well-defined experimental preparation for the study of ligand-dependant synaptic transmission. Its large size gives direct experimental access to both presynaptic and postsynaptic junctional ...
  • Orientation-independent differential interference contrast microscopy 

    Shribak, Michael; Inoue, Shinya (2006)
    The image in a regular DIC microscope reflects the orientation of the prism shear direction and the optical path gradients in a phase specimen. If the shear direction lies parallel to the specimen boundary no contrast ...
  • Pac-man motility of kinetochores unleashed by laser microsurgery 

    LaFountain, James R.; Cohan, Christopher S.; Oldenbourg, Rudolf (American Society for Cell Biology, 2012-06-27)
    We report on experiments directly in living cells that reveal the regulation of kinetochore function by tension. X and Y sex chromosomes in crane fly (Nephrotoma suturalis) spermatocytes exhibit an atypical segregation ...