Protein methylation in full length Chlamydomonas flagella
MetadataShow full item record
Post-translational protein modification occurs extensively in eukaryotic flagella. Here we examine protein methylation, a protein modification that has only recently been reported to occur in flagella (Schneider et al. 2008). The cobalamin (vitamin B12) independent form of the enzyme methionine synthase (MetE), which catalyzes the final step in methionine production, is localized to flagella. Here we demonstrate, using immunogold scanning electron microscopy, that MetE is bound to the outer doublets of the flagellum. Methionine can be converted to Sadenosyl methionine, which then serves as the methyl donor for protein methylation reactions. Using antibodies that recognize symmetrically or asymmetrically methylated arginine residues, we identify three highly methylated proteins in intact flagella: two symmetrically methylated proteins of about 30 and 40 kDa, and one asymmetrically methylated protein of about 75 kDa. Several other relatively less methylated proteins could also be detected. Fractionation and immunoblot analysis shows that these proteins are components of the flagellar axoneme. Immunogold thin section electron microscopy indicates that the symmetrically methylated proteins are located in the central region of the axoneme, perhaps as components of the central pair complex and the radial spokes, while the asymmetrically methylated proteins are associated with the outer doublets.
Author Posting. © The Author(s), 2009. This is the author's version of the work. It is posted here by permission of John Wiley & Sons for personal use, not for redistribution. The definitive version was published in Cell Motility and the Cytoskeleton 66 (2009): 650-660, doi:10.1002/cm.20387.
Suggested CitationPreprint: Sloboda, Roger D., Howard, Louisa, "Protein methylation in full length Chlamydomonas flagella", 2009-04-28, https://doi.org/10.1002/cm.20387, https://hdl.handle.net/1912/2967
Showing items related by title, author, creator and subject.
Schneider, Mark J.; Ulland, Megan; Sloboda, Roger D. (American Society for Cell Biology, 2008-08-13)During intraflagellar transport (IFT), the regulation of motor proteins, the loading and unloading of cargo and the turnover of flagellar proteins all occur at the flagellar tip. To begin an analysis of the protein composition ...
Characterization of methyl sugars, 3-deoxysugars and methyl deoxysugars in marine high molecular weight dissolved organic matter Panagiotopoulos, Christos; Repeta, Daniel J.; Johnson, Carl G. (2007-01-16)Nuclear magnetic resonance spectroscopy of marine high molecular weight dissolved organic matter (HMWDOM) in surface waters show that >50% of the carbon is a compositionally well-defined family of acylated-polysaccharides ...
Pangallo, Kristin C. (Massachusetts Institute of Technology and Woods Hole Oceanographic Institution, 2009-09)Halogenated 1’-methyl-1,2’-bipyrroles (MBPs) are a distinctive class of marine organic compounds. They are naturally produced, they have a unique carbon structure, they are highly halogenated, and they bioaccumulate in ...