Distribution of postsynaptic density (PSD)-95 and Ca2+/calmodulin-dependent protein kinase II at the PSD


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dc.contributor.author Petersen, Jennifer D.
dc.contributor.author Chen, Xiaobing
dc.contributor.author Vinade, Lucia
dc.contributor.author Dosemeci, Ayse
dc.contributor.author Lisman, John E.
dc.contributor.author Reese, Thomas S.
dc.date.accessioned 2009-08-25T19:02:58Z
dc.date.available 2009-08-25T19:02:58Z
dc.date.issued 2003-12-03
dc.identifier.citation Journal of Neuroscience 23 (2003): 11270-11278 en
dc.identifier.uri http://hdl.handle.net/1912/2962
dc.description Author Posting. © Society for Neuroscience, 2003. This article is posted here by permission of Society for Neuroscience for personal use, not for redistribution. The definitive version was published in Journal of Neuroscience 23 (2003): 11270-11278. en
dc.description.abstract Postsynaptic densities (PSDs) contain proteins that regulate synaptic transmission. We determined the positions of calcium/calmodulin-dependent protein kinase II (CaMKII) and PSD-95 within the three-dimensional structure of isolated PSDs using immunogold labeling, rotary shadowing, and electron microscopic tomography. The results show that all PSDs contain a central mesh immediately underlying the postsynaptic membrane. Label for PSD-95 is found on both the cytoplasmic and cleft sides of this mesh, averaging 12 nm from the cleft side. All PSDs label for PSD-95. The properties of CaMKII labeling are quite different. Label is virtually absent on the cleft sides of PSDs, but can be heavy on the cytoplasmic side at a mean distance of 25 nm from the cleft. In tomograms, CaMKII holoenzymes can be visualized directly, appearing as labeled, tower-like structures reflecting the 20 nm diameter of the holoenzyme. These towers protrude from the cytoplasmic side of the central mesh. There appears to be a local organization of CaMKII, as judged by fact that the nearest-neighbor distances are nearly invariant over a wide range of labeling density for CaMKII. The average density of CaMKII holoenzymes is highly variable, ranging from zero to values approaching a tightly packed state. This variability is significantly higher than that for PSD-95 and is consistent with an information storage role for CaMKII. en
dc.description.sponsorship This work was supported by National Institute of Neurological Disorders and Stroke Grants RO1 NS-27337 and RO1 NS-35083 (J.L.). en
dc.format.mimetype application/pdf
dc.language.iso en_US en
dc.publisher Society for Neuroscience en
dc.relation.uri http://www.jneurosci.org/cgi/content/full/23/35/11270
dc.subject Postsynaptic density en
dc.subject CaMKII en
dc.subject PSD en
dc.subject PSD-95 en
dc.subject Electron microscopy en
dc.subject Tomography en
dc.title Distribution of postsynaptic density (PSD)-95 and Ca2+/calmodulin-dependent protein kinase II at the PSD en
dc.type Article en

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