Distribution of postsynaptic density (PSD)-95 and Ca2+/calmodulin-dependent protein kinase II at the PSD
Petersen, Jennifer D.
Lisman, John E.
Reese, Thomas S.
MetadataShow full item record
Postsynaptic densities (PSDs) contain proteins that regulate synaptic transmission. We determined the positions of calcium/calmodulin-dependent protein kinase II (CaMKII) and PSD-95 within the three-dimensional structure of isolated PSDs using immunogold labeling, rotary shadowing, and electron microscopic tomography. The results show that all PSDs contain a central mesh immediately underlying the postsynaptic membrane. Label for PSD-95 is found on both the cytoplasmic and cleft sides of this mesh, averaging 12 nm from the cleft side. All PSDs label for PSD-95. The properties of CaMKII labeling are quite different. Label is virtually absent on the cleft sides of PSDs, but can be heavy on the cytoplasmic side at a mean distance of 25 nm from the cleft. In tomograms, CaMKII holoenzymes can be visualized directly, appearing as labeled, tower-like structures reflecting the 20 nm diameter of the holoenzyme. These towers protrude from the cytoplasmic side of the central mesh. There appears to be a local organization of CaMKII, as judged by fact that the nearest-neighbor distances are nearly invariant over a wide range of labeling density for CaMKII. The average density of CaMKII holoenzymes is highly variable, ranging from zero to values approaching a tightly packed state. This variability is significantly higher than that for PSD-95 and is consistent with an information storage role for CaMKII.
Author Posting. © Society for Neuroscience, 2003. This article is posted here by permission of Society for Neuroscience for personal use, not for redistribution. The definitive version was published in Journal of Neuroscience 23 (2003): 11270-11278.
Suggested CitationArticle: Petersen, Jennifer D., Chen, Xiaobing, Vinade, Lucia, Dosemeci, Ayse, Lisman, John E., Reese, Thomas S., "Distribution of postsynaptic density (PSD)-95 and Ca2+/calmodulin-dependent protein kinase II at the PSD", Journal of Neuroscience 23 (2003): 11270-11278, https://hdl.handle.net/1912/2962
Showing items related by title, author, creator and subject.
An inverse method for obtaining the attenuation profile and small variations in the sound speed and density profiles of the ocean bottom Rajan, Subramaniam D. (Massachusetts Institute of Technology and Woods Hole Oceanographic Institution, 1985-05)The acoustic properties of marine sediments have a direct effect on the propagation of sound in the ocean. In the frequency range of interest (50 - 500 Hz) the sediment can be modelled as a fluid. Assuming horizontal ...
Benthic foraminifera living in Gulf of Mexico bathyal and abyssal sediments : community analysis and comparison to metazoan meiofaunal biomass and density Bernhard, Joan M.; Sen Gupta, Barun K.; Baguley, Jeffrey G. (2005-12-07)Benthic foraminiferal biomass, density, and species composition were determined at ten sites in the Gulf of Mexico. During June 2001 and June 2002, sediment samples were collected with a GoMex boxcorer. A 7.5-cm diameter ...
High-frequency acoustic scattering from turbulent oceanic microstructure : the importance of density fluctuations Lavery, Andone C.; Schmitt, Raymond W.; Stanton, Timothy K. (Acoustical Society of America, 2003-11)Acoustic scattering techniques provide a unique and powerful tool to remotely investigate the physical properties of the ocean interior over large spatial and temporal scales. With high-frequency acoustic scattering it is ...