Distribution of postsynaptic density (PSD)-95 and Ca2+/calmodulin-dependent protein kinase II at the PSD
Petersen, Jennifer D.
Lisman, John E.
Reese, Thomas S.
MetadataShow full item record
Postsynaptic densities (PSDs) contain proteins that regulate synaptic transmission. We determined the positions of calcium/calmodulin-dependent protein kinase II (CaMKII) and PSD-95 within the three-dimensional structure of isolated PSDs using immunogold labeling, rotary shadowing, and electron microscopic tomography. The results show that all PSDs contain a central mesh immediately underlying the postsynaptic membrane. Label for PSD-95 is found on both the cytoplasmic and cleft sides of this mesh, averaging 12 nm from the cleft side. All PSDs label for PSD-95. The properties of CaMKII labeling are quite different. Label is virtually absent on the cleft sides of PSDs, but can be heavy on the cytoplasmic side at a mean distance of 25 nm from the cleft. In tomograms, CaMKII holoenzymes can be visualized directly, appearing as labeled, tower-like structures reflecting the 20 nm diameter of the holoenzyme. These towers protrude from the cytoplasmic side of the central mesh. There appears to be a local organization of CaMKII, as judged by fact that the nearest-neighbor distances are nearly invariant over a wide range of labeling density for CaMKII. The average density of CaMKII holoenzymes is highly variable, ranging from zero to values approaching a tightly packed state. This variability is significantly higher than that for PSD-95 and is consistent with an information storage role for CaMKII.
Author Posting. © Society for Neuroscience, 2003. This article is posted here by permission of Society for Neuroscience for personal use, not for redistribution. The definitive version was published in Journal of Neuroscience 23 (2003): 11270-11278.
Suggested CitationArticle: Petersen, Jennifer D., Chen, Xiaobing, Vinade, Lucia, Dosemeci, Ayse, Lisman, John E., Reese, Thomas S., "Distribution of postsynaptic density (PSD)-95 and Ca2+/calmodulin-dependent protein kinase II at the PSD", Journal of Neuroscience 23 (2003): 11270-11278, https://hdl.handle.net/1912/2962
Showing items related by title, author, creator and subject.
Density currents in the Chicago River : characterization, effects on water quality, and potential sources Jackson, P. Ryan; Garcia, Carlos M.; Oberg, Kevin A.; Johnson, Kevin K.; Garcia, Marcelo H. (2008-03)Bidirectional flows in a river system can occur under stratified flow conditions and in addition to creating significant errors in discharge estimates, the upstream propagating currents are capable of transporting contaminants ...
Evaluation of an acoustic remote sensing method for frontal-zone studies using double-diffusive instability microstructure data and density interface data from intrusions Duda, Timothy F.; Lavery, Andone C.; Sellers, Cynthia J. (Elsevier, 2016-10-19)Understanding intrusive exchange at oceanic water mass fronts may depend on building data-constrained models of the processes, but obtaining the needed representative and comprehensive data is challenging. Acoustic imaging ...
Sullivan, Lauren L.; Li, Bingtuan; Miller, Tom E. X.; Neubert, Michael G.; Shaw, Allison K. (2017-04)Mitigating the spread of invasive species remains difficult—substantial variability in invasion speed is increasingly well-documented, but the sources of this variability are poorly understood. We report a mechanism for ...