Voltage imaging from dendrites of mitral cells : EPSP attenuation and spike trigger zones
Chen, Wei R.
MetadataShow full item record
KeywordMitral cells; Voltage imaging; Voltage-sensitive dyes; Dendrites; Synaptic integration; Olfaction
To obtain a more complete description of individual neurons, it is necessary to complement the electrical patch pipette measurements with technologies that permit a massive parallel recording from many sites on neuronal processes. This can be achieved by using voltage imaging with intracellular dyes. With this approach, we investigated the functional structure of a mitral cell, the principal output neuron in the rat olfactory bulb. The most significant finding concerns the characteristics of EPSPs at the synaptic sites and surprisingly small attenuation along the trunk of the primary dendrite. Also, the experiments were performed to determine the number, location, and stability of spike trigger zones, the excitability of terminal dendritic branches, and the pattern and nature of spike initiation and propagation in the primary and secondary dendrites. The results show that optical data can be used to deduce the amplitude and shape of the EPSPs evoked by olfactory nerve stimulation at the site of origin (glomerular tuft) and to determine its attenuation along the entire length of the primary dendrite. This attenuation corresponds to an unusually large mean apparent "length constant" of the primary dendrite. Furthermore, the images of spike trigger zones showed that an action potential can be initiated in three different compartments of the mitral cell: the soma-axon region, the primary dendrite trunk, and the terminal dendritic tuft, which appears to be fully excitable. Finally, secondary dendrites clearly support the active propagation of action potentials
Author Posting. © Society for Neuroscience, 2004. This article is posted here by permission of Society for Neuroscience for personal use, not for redistribution. The definitive version was published in Journal of Neuroscience 24 (2004): 6703-6714, doi:10.1523/JNEUROSCI.0307-04.2004.
Showing items related by title, author, creator and subject.
Synaptically activated Ca2+ waves and NMDA spikes locally suppress voltage-dependent Ca2+ signalling in rat pyramidal cell dendrites Manita, Satoshi; Miyazaki, Kenichi; Ross, William N. (2011-08)Synaptically activated changes in dendritic [Ca2+]i affect many important physiological processes including synaptic plasticity and gene expression. The location, magnitude, and time course of these changes can determine ...
Dendritic signals from rat hippocampal CA1 pyramidal neurons during coincident pre- and post-synaptic activity : a combined voltage- and calcium-imaging study Canepari, Marco; Djurisic, Maja; Zecevic, Dejan (2006-11-16)The non-linear and spatially inhomogeneous interactions of dendritic membrane potential signals that represent the first step in the induction of activity dependent long-term synaptic plasticity are not fully understood, ...
Developmental profile of localized spontaneous Ca2+ release events in the dendrites of rat hippocampal pyramidal neurons Miyazaki, Kenichi; Manita, Satoshi; Ross, William N. (2012-08)Recent experiments demonstrate that localized spontaneous Ca2+ release events can be detected in the dendrites of pyramidal cells in the hippocampus and other neurons (J. Neurosci. 29:7833-7845, 2009). These events have ...