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Imaging the division process in living tissue culture cells

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dc.contributor.author Khodjakov, Alexey
dc.contributor.author Rieder, Conly L.
dc.date.accessioned 2005-12-21T15:21:56Z
dc.date.available 2005-12-21T15:21:56Z
dc.date.issued 2005-07-29
dc.identifier.uri http://hdl.handle.net/1912/292
dc.description Author Posting. © The Authors, 2005. This is the author's version of the work. It is posted here by permission of Elsevier B. V. for personal use, not for redistribution. The definitive version was published in Methods 38 (2006): 2-16, doi:10.1016/j.ymeth.2005.07.007.
dc.description.abstract We detail some of the pitfalls encountered when following live cultured somatic cells by light microscopy during mitosis. Principle difficulties in this methodology arise from the necessity to compromise between maintaining the health of the cell while achieving the appropriate temporal and spatial resolutions required for the study. Although the quality of the data collected from fixed cells is restricted only by the quality of the imaging system and the optical properties of the specimen, the major limiting factor when viewing live cells is radiation damage induced during illumination. We discuss practical considerations for minimizing this damage, and for maintaining the general health of the cell, while it is being followed by multi-mode or multi-dimensional light microscopy. en
dc.format.extent 845552 bytes
dc.format.mimetype application/pdf
dc.language.iso en_US en
dc.relation.uri http://dx.doi.org/10.1016/j.ymeth.2005.07.007
dc.title Imaging the division process in living tissue culture cells en
dc.type Preprint en


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