Discovery of chlorophyll d: isolation and characterization of a far-red cyanobacterium from the original site of manning and strain (1943) at Moss Beach, California
Kiang, Nancy Y.
Swingley, Wesley D.
Broddrick, Jared T.
Repeta, Daniel J.
Stolz, John F.
Blankenship, Robert E.
Wolf, Benjamin M.
Detweiler, Angela M.
Miller, Kathy Ann
Schladweiler, Jacob J.
Parenteau, Mary N.
MetadataShow full item record
KeywordChlorophyll d; Acaryochloris; Moss Beach; Cyanobacteria; Far-red photosynthesis; Photosynthetic pigments; Absorbance spectra; Genome sequence
We have isolated a chlorophyll-d-containing cyanobacterium from the intertidal field site at Moss Beach, on the coast of Central California, USA, where Manning and Strain (1943) originally discovered this far-red chlorophyll. Here, we present the cyanobacterium’s environmental description, culturing procedure, pigment composition, ultrastructure, and full genome sequence. Among cultures of far-red cyanobacteria obtained from red algae from the same site, this strain was an epiphyte on a brown macroalgae. Its Qyin vivo absorbance peak is centered at 704–705 nm, the shortest wavelength observed thus far among the various known Acaryochloris strains. Its Chl a/Chl d ratio was 0.01, with Chl d accounting for 99% of the total Chl d and Chl a mass. TEM imagery indicates the absence of phycobilisomes, corroborated by both pigment spectra and genome analysis. The Moss Beach strain codes for only a single set of genes for producing allophycocyanin. Genomic sequencing yielded a 7.25 Mbp circular chromosome and 10 circular plasmids ranging from 16 kbp to 394 kbp. We have determined that this strain shares high similarity with strain S15, an epiphyte of red algae, while its distinct gene complement and ecological niche suggest that this strain could be the closest known relative to the original Chl d source of Manning and Strain (1943). The Moss Beach strain is designated Acaryochloris sp. (marina) strain Moss Beach.
© The Author(s), 2022. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Kiang, N. Y., Swingley, W. D., Gautam, D., Broddrick, J. T., Repeta, D. J., Stolz, J. F., Blankenship, R. E., Wolf, B. M., Detweiler, A. M., Miller, K. A., Schladweiler, J. J., Lindeman, R., & Parenteau, M. N. Discovery of chlorophyll d: isolation and characterization of a far-red cyanobacterium from the original site of manning and strain (1943) at Moss Beach, California. Microorganisms, 10(4), (2022): 819, https://doi.org/10.3390/microorganisms10040819.
Suggested CitationKiang, N. Y., Swingley, W. D., Gautam, D., Broddrick, J. T., Repeta, D. J., Stolz, J. F., Blankenship, R. E., Wolf, B. M., Detweiler, A. M., Miller, K. A., Schladweiler, J. J., Lindeman, R., & Parenteau, M. N. (2022). Discovery of chlorophyll d: isolation and characterization of a far-red cyanobacterium from the original site of manning and strain (1943) at Moss Beach, California. Microorganisms, 10(4), 819.
The following license files are associated with this item:
Showing items related by title, author, creator and subject.
Nutrient and chlorophyll analyses from surface samples collected underway on board container ships during basin-wide transects of the North Pacific Ocean from Hong Kong to Long Beach, CA from 2009-2012 Palevsky, Hilary I.; Quay, Paul (Biological and Chemical Oceanography Data Management Office (BCO-DMO). Contact: firstname.lastname@example.org, 2021-07-06)Surface nutrient and chlorophyll analyses were performed on samples collected on board commercial container ships from April 2009 through December 2012. Samples were collected from the underway seawater system during ...
Beach changes and management options for Nauset Barrier Beach and Orleans Town Beach, Cape Cod, MA : report to the Town of Orleans Aubrey, David G.; Robertson, William (Woods Hole Oceanographic Institution, 1998-04)The present study investigated the shoreline and dune changes occurrng along Nauset Barrier Beach system during the past sixty years, based on examination of aerial photographs, charts, and other data sources. Significant ...
Aubrey, David G.; Gaines, Arthur G. (Woods Hole Oceanographic Institution, 1982-01)Popponesset Spit, the barrier beach sheltering Popponesset Bay on Cape Cod, Massachusetts, has experienced large changes in its location and shape over the past thirty years. Concern by the public over loss of this ...