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dc.contributor.authorReitzel, Adam M.  Concept link
dc.contributor.authorSullivan, James C.  Concept link
dc.contributor.authorTraylor-Knowles, Nikki  Concept link
dc.contributor.authorFinnerty, John R.  Concept link
dc.date.accessioned2009-06-03T18:33:27Z
dc.date.available2009-06-03T18:33:27Z
dc.date.issued2008-06
dc.identifier.citationBiological Bulletin 214 (2008): 233-254en
dc.identifier.urihttps://hdl.handle.net/1912/2845
dc.descriptionAuthor Posting. © Marine Biological Laboratory, 2008. This article is posted here by permission of Marine Biological Laboratory for personal use, not for redistribution. The definitive version was published in Biological Bulletin 214 (2008): 233-254.en
dc.description.abstractSalt marshes are challenging habitats due to natural variability in key environmental parameters including temperature, salinity, ultraviolet light, oxygen, sulfides, and reactive oxygen species. Compounding this natural variation, salt marshes are often heavily impacted by anthropogenic insults including eutrophication, toxic contamination, and coastal development that alter tidal and freshwater inputs. Commensurate with this environmental variability, estuarine animals generally exhibit broader physiological tolerances than freshwater, marine, or terrestrial species. One factor that determines an organism's physiological tolerance is its ability to upregulate "stress-response genes" in reaction to particular stressors. Comparative studies on diverse organisms have identified a number of evolutionarily conserved genes involved in responding to abiotic and biotic stressors. We used homology-based scans to survey the sequenced genome of Nematostella vectensis, the starlet sea anemone, an estuarine specialist, to identify genes involved in the response to three kinds of insult—physiochemical insults, pathogens, and injury. Many components of the stress-response networks identified in triploblastic animals have clear orthologs in the sea anemone, meaning that they must predate the cnidarian-triploblast split (e.g., xenobiotic receptors, biotransformative genes, ATP-dependent transporters, and genes involved in responding to reactive oxygen species, toxic metals, osmotic shock, thermal stress, pathogen exposure, and wounding). However, in some instances, stress-response genes known from triploblasts appear to be absent from the Nematostella genome (e.g., many metal-complexing genes). This is the first comprehensive examination of the genomic stress-response repertoire of an estuarine animal and a member of the phylum Cnidaria. The molecular markers of stress response identified in Nematostella may prove useful in monitoring estuary health and evaluating coastal conservation efforts. These data may also inform conservation efforts on other cnidarians, such as the reef-building corals.en
dc.description.sponsorshipAMR was supported by a Postdoctoral Scholar Program at the Woods Hole Oceanographic Institution, with funding provided by The Beacon Institute for Rivers and Estuaries, and the J. Seward Johnson Fund. NTK was supported by a graduate research training grant from the National Institutes of Health. This research was also supported by NSF grant FP-91656101-0 to JCS and JRF, EPA grant F5E11155 to AMR and JRF, and a grant from the Conservation International Marine Management Area Science Program to JRF.en
dc.format.mimetypeapplication/pdf
dc.language.isoen_USen
dc.publisherMarine Biological Laboratoryen
dc.relation.urihttps://doi.org/10.2307/25470666
dc.subjectECM, extracellular matrixen
dc.subjectEST, expressed sequence tagen
dc.subjectROS, reactive oxygen speciesen
dc.titleGenomic survey of candidate stress-response genes in the estuarine anemone Nematostella vectensisen
dc.typeArticleen
dc.identifier.doi10.2307/25470666


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