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dc.contributor.authorVoordeckers, James W.  Concept link
dc.contributor.authorDo, My H.  Concept link
dc.contributor.authorHugler, Michael  Concept link
dc.contributor.authorKo, Vivian  Concept link
dc.contributor.authorSievert, Stefan M.  Concept link
dc.contributor.authorVetriani, Costantino  Concept link
dc.date.accessioned2009-06-03T18:03:43Z
dc.date.available2009-06-03T18:03:43Z
dc.date.issued2008-04
dc.identifier.urihttps://hdl.handle.net/1912/2843
dc.descriptionAuthor Posting. © Springer, 2008. This is the author's version of the work. It is posted here by permission of Springer for personal use, not for redistribution. The definitive version was published in Extremophiles 12 (2008): 627-640, doi:10.1007/s00792-008-0167-5.en
dc.description.abstractThe bacterial and archaeal communities of three deep-sea hydrothermal vent systems located on the Mid-Atlantic Ridge (MAR; Rainbow, Logatchev and Broken Spur) were investigated using an integrated culture-dependent and independent approach. Comparative molecular phylogenetic analyses, using the 16S rRNA gene and the deduced amino acid sequences of the alpha and beta subunits of the ATP citrate lyase encoding genes were carried out on natural microbial communities, on an enrichment culture obtained from the Broken Spur chimney, and on novel chemolithoautotrophic bacteria and reference strains originally isolated from several different deep-sea vents. Our data showed that the three MAR hydrothermal vent chimneys investigated in this study host very different microbial assemblages. The microbial community of the Rainbow chimney was dominated by thermophilic, autotrophic, hydrogen-oxidizing, sulfur- and nitrate reducing Epsilonproteobacteria related to the genus Caminibacter. The detection of sequences related to sulfur-reducing bacteria and archaea (Archaeoglobus) indicated that thermophilic sulfate reduction might also be occurring at this site. The Logatchev bacterial community included several sequences related to mesophilic sulfur-oxidizing bacteria, while the archaeal component of this chimney was dominated by sequences related to the ANME-2 lineage, suggesting that anaerobic oxidation of methane may be occurring at this site. Comparative analyses of the ATP citrate lyase encoding genes from natural microbial communities suggested that Epsilonproteobacteria were the dominant primary producers using the reverse TCA cycle (rTCA) at Rainbow, while Aquificales of the genera Desulfurobacterium and Persephonella were prevalent in the Broken Spur chimney.en
dc.description.sponsorshipThis research was supported by NSF grants MCB 04-56676 (C.V.), OCE 03-27353 (C.V.), MCB 04-56689 (S.M.S.), a grant from the New Jersey Agricultural Experiment Station to C.V., and a NIH Ph.D. Training Program in Biotechnology Fellowship (NIH NIGMS 5 T32 GM08339) to J.V. M.H. was supported through a postdoctoral scholarship from the Woods Hole Oceanographic Institution.en
dc.format.mimetypeapplication/pdf
dc.language.isoen_USen
dc.relation.urihttps://doi.org/10.1007/s00792-008-0167-5
dc.subjectEpsilonproteobacteriaen
dc.subjectAquificalesen
dc.subjectANMEen
dc.subjectATP citrate lyaseen
dc.subjectBlack smokeren
dc.subjectDeep-sea venten
dc.subjectrTCA cycleen
dc.titleCulture dependent and independent analyses of 16S rRNA and ATP citrate lyase genes : a comparison of microbial communities from different black smoker chimneys on the Mid-Atlantic Ridgeen
dc.typePreprinten


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