Culture dependent and independent analyses of 16S rRNA and ATP citrate lyase genes : a comparison of microbial communities from different black smoker chimneys on the Mid-Atlantic Ridge
Voordeckers, James W.
Do, My H.
Sievert, Stefan M.
MetadataShow full item record
KeywordEpsilonproteobacteria; Aquificales; ANME; ATP citrate lyase; Black smoker; Deep-sea vent; rTCA cycle
The bacterial and archaeal communities of three deep-sea hydrothermal vent systems located on the Mid-Atlantic Ridge (MAR; Rainbow, Logatchev and Broken Spur) were investigated using an integrated culture-dependent and independent approach. Comparative molecular phylogenetic analyses, using the 16S rRNA gene and the deduced amino acid sequences of the alpha and beta subunits of the ATP citrate lyase encoding genes were carried out on natural microbial communities, on an enrichment culture obtained from the Broken Spur chimney, and on novel chemolithoautotrophic bacteria and reference strains originally isolated from several different deep-sea vents. Our data showed that the three MAR hydrothermal vent chimneys investigated in this study host very different microbial assemblages. The microbial community of the Rainbow chimney was dominated by thermophilic, autotrophic, hydrogen-oxidizing, sulfur- and nitrate reducing Epsilonproteobacteria related to the genus Caminibacter. The detection of sequences related to sulfur-reducing bacteria and archaea (Archaeoglobus) indicated that thermophilic sulfate reduction might also be occurring at this site. The Logatchev bacterial community included several sequences related to mesophilic sulfur-oxidizing bacteria, while the archaeal component of this chimney was dominated by sequences related to the ANME-2 lineage, suggesting that anaerobic oxidation of methane may be occurring at this site. Comparative analyses of the ATP citrate lyase encoding genes from natural microbial communities suggested that Epsilonproteobacteria were the dominant primary producers using the reverse TCA cycle (rTCA) at Rainbow, while Aquificales of the genera Desulfurobacterium and Persephonella were prevalent in the Broken Spur chimney.
Author Posting. © Springer, 2008. This is the author's version of the work. It is posted here by permission of Springer for personal use, not for redistribution. The definitive version was published in Extremophiles 12 (2008): 627-640, doi:10.1007/s00792-008-0167-5.
Suggested CitationPreprint: Voordeckers, James W., Do, My H., Hugler, Michael, Ko, Vivian, Sievert, Stefan M., Vetriani, Costantino, "Culture dependent and independent analyses of 16S rRNA and ATP citrate lyase genes : a comparison of microbial communities from different black smoker chimneys on the Mid-Atlantic Ridge", 2008-04, https://doi.org/10.1007/s00792-008-0167-5, https://hdl.handle.net/1912/2843
Showing items related by title, author, creator and subject.
Temporal and spatial variability in sedimentation in the Black Sea : cruise report R/V Knorr 134-8, Black Sea Leg 1, April 16-May 7, 1988 Honjo, Susumu; Hay, Bernward J.; Members of the Scientific Shipboard Party (Woods Hole Oceanographic Institution, 1988-10)This document represents the cruise report of the highly successful Leg 1 of the R/V Knorr cruise to the Black Sea (Cruise 134-8) as a joint Turkish-American Oceanographic Expedition (Izmir to Istanbul, April 16 to May ...
Ancient DNA derived from alkenone-biosynthesizing haptophytes and other algae in Holocene sediments from the Black Sea Coolen, Marco J. L.; Boere, Arjan C.; Abbas, Ben; Baas, Marianne; Wakeham, Stuart G.; Sinninghe Damste, Jaap S. (American Geophysical Union, 2006-02-18)Holocene sea surface temperatures (SST) of the Black Sea have been reconstructed using sedimentary C37 unsaturated alkenones assumed to be derived from the coccolithophorid haptophyte Emiliania huxleyi, whose fossil ...
Giosan, Liviu; Coolen, Marco J. L.; Kaplan, Jed O.; Constantinescu, Stefan; Filip, Florin; Filipova-Marinova, Mariana; Kettner, Albert J.; Thom, Nick (Nature Publishing Group, 2012-08-30)Over the last century humans have altered the export of fluvial materials leading to significant changes in morphology, chemistry, and biology of the coastal ocean. Here we present sedimentary, paleoenvironmental and ...