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dc.contributor.authorVaezeslami, Soheila  Concept link
dc.contributor.authorSterling, Rachel  Concept link
dc.contributor.authorReznikoff, William S.  Concept link
dc.date.accessioned2009-05-12T15:38:20Z
dc.date.available2009-05-12T15:38:20Z
dc.date.issued2007-08-10
dc.identifier.citationJournal of Bacteriology 189 (2007): 7436-7441en
dc.identifier.urihttps://hdl.handle.net/1912/2827
dc.descriptionAuthor Posting. © American Society for Microbiology, 2007. This article is posted here by permission of American Society for Microbiology for personal use, not for redistribution. The definitive version was published in Journal of Bacteriology 189 (2007): 7436-7441, doi:10.1128/JB.00524-07.en
dc.description.abstractTransposition (the movement of discrete segments of DNA, resulting in rearrangement of genomic DNA) initiates when transposase forms a dimeric DNA-protein synaptic complex with transposon DNA end sequences. The synaptic complex is a prerequisite for catalytic reactions that occur during the transposition process. The transposase-DNA interactions involved in the synaptic complex have been of great interest. Here we undertook a study to verify the protein-DNA interactions that lead to synapsis in the Tn5 system. Specifically, we studied (i) Arg342, Glu344, and Asn348 and (ii) Ser438, Lys439, and Ser445, which, based on the previously published cocrystal structure of Tn5 transposase bound to a precleaved transposon end sequence, make cis and trans contacts with transposon end sequence DNA, respectively. By using genetic and biochemical assays, we showed that in all cases except one, each of these residues plays an important role in synaptic complex formation, as predicted by the cocrystal structure.en
dc.description.sponsorshipThis work was supported by NIH grant GM 50692.en
dc.format.mimetypeapplication/pdf
dc.language.isoen_USen
dc.publisherAmerican Society for Microbiologyen
dc.relation.urihttps://doi.org/10.1128/JB.00524-07
dc.titleSite-directed mutagenesis studies of Tn5 transposase residues involved in synaptic complex formationen
dc.typeArticleen
dc.identifier.doi10.1128/JB.00524-07


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