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    Characterization of cytochrome 579, an unusual cytochrome isolated from an iron-oxidizing microbial community

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    4454.pdf (1.242Mb)
    Date
    2008-05-09
    Author
    Singer, Steven W.  Concept link
    Chan, Clara S.  Concept link
    Zemla, Adam  Concept link
    VerBerkmoes, Nathan C.  Concept link
    Hwang, Mona  Concept link
    Hettich, Robert L.  Concept link
    Banfield, Jillian F.  Concept link
    Thelen, Michael P.  Concept link
    Metadata
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    Citable URI
    https://hdl.handle.net/1912/2823
    As published
    https://doi.org/10.1128/AEM.02799-07
    DOI
    10.1128/AEM.02799-07
    Abstract
    A novel, soluble cytochrome with an unusual visible spectral signature at 579 nm (Cyt579) has been characterized after isolation from several different microbial biofilms collected in an extremely acidic ecosystem. Previous proteogenomic studies of an Fe(II)-oxidizing community indicated that this abundant red cytochrome could be extracted from the biofilms with dilute sulfuric acid. Here, we found that the Fe(II)-dependent reduction of Cyt579 was thermodynamically favorable at a pH of >3, raising the possibility that Cyt579 acts as an accessory protein for electron transfer. The results of transmission electron microscopy of immunogold-labeled biofilm indicated that Cyt579 is localized near the bacterial cell surface, consistent with periplasmic localization. The results of further protein analysis of Cyt579, using preparative chromatofocusing and sodium dodecyl sulfate-polyacrylamide gel electrophoresis, revealed three forms of the protein that correspond to different N-terminal truncations of the amino acid sequence. The results of intact-protein analysis corroborated the posttranslational modifications of these forms and identified a genomically uncharacterized Cyt579 variant. Homology modeling was used to predict the overall cytochrome structure and heme binding site; the positions of nine amino acid substitutions found in three Cyt579 variants all map to the surface of the protein and away from the heme group. Based on this detailed characterization of Cyt579, we propose that Cyt579 acts as an electron transfer protein, shuttling electrons derived from Fe(II) oxidation to support critical metabolic functions in the acidophilic microbial community.
    Description
    Author Posting. © American Society for Microbiology, 2008. This article is posted here by permission of American Society for Microbiology for personal use, not for redistribution. The definitive version was published in Applied and Environmental Microbiology 74 (2008): 4454-4462, doi:10.1128/AEM.02799-07.
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    • Marine Chemistry and Geochemistry (MC&G)
    Suggested Citation
    Applied and Environmental Microbiology 74 (2008): 4454-4462
     

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