• Login
    About WHOAS
    View Item 
    •   WHOAS Home
    • Marine Biological Laboratory
    • Josephine Bay Paul Center in Comparative Molecular Biology and Evolution
    • View Item
    •   WHOAS Home
    • Marine Biological Laboratory
    • Josephine Bay Paul Center in Comparative Molecular Biology and Evolution
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of WHOASCommunities & CollectionsBy Issue DateAuthorsTitlesKeywordsThis CollectionBy Issue DateAuthorsTitlesKeywords

    My Account

    LoginRegister

    Statistics

    View Usage Statistics

    Human high density lipoproteins are platforms for the assembly of multi-component innate immune complexes

    Thumbnail
    View/Open
    32578.pdf (500.5Kb)
    Date
    2005-07-26
    Author
    Shiflett, April M.  Concept link
    Bishop, Joseph R.  Concept link
    Pahwa, Amit  Concept link
    Hajduk, Stephen L.  Concept link
    Metadata
    Show full item record
    Citable URI
    https://hdl.handle.net/1912/2811
    As published
    https://doi.org/10.1074/jbc.M503510200
    DOI
    10.1074/jbc.M503510200
    Abstract
    Human innate immunity to non-pathogenic species of African trypanosomes is provided by human high density lipoprotein (HDL) particles. Here we show that native human HDLs containing haptoglobin-related protein (Hpr), apolipoprotein L-I (apoL-I) and apolipoprotein A-I (apoA-I) are the principle antimicrobial molecules providing protection from trypanosome infection. Other HDL subclasses containing either apoA-I and apoL-I or apoA-I and Hpr have reduced trypanolytic activity, whereas HDL subclasses lacking apoL-I and Hpr are non-toxic to trypanosomes. Highly purified, lipid-free Hpr and apoL-I were both toxic to Trypanosoma brucei brucei but with specific activities at least 500-fold less than those of native HDLs, suggesting that association of these apolipoproteins within the HDL particle was necessary for optimal cytotoxicity. These studies show that HDLs can serve as platforms for the assembly of multiple synergistic proteins and that these assemblies may play a critical role in the evolution of primate-specific innate immunity to trypanosome infection.
    Description
    Author Posting. © American Society for Biochemistry and Molecular Biology, 2005. This article is posted here by permission of American Society for Biochemistry and Molecular Biology for personal use, not for redistribution. The definitive version was published in Journal of Biological Chemistry 280 (2005): 32578-3258, doi:10.1074/jbc.M503510200.
    Collections
    • Josephine Bay Paul Center in Comparative Molecular Biology and Evolution
    Suggested Citation
    Article: Shiflett, April M., Bishop, Joseph R., Pahwa, Amit, Hajduk, Stephen L., "Human high density lipoproteins are platforms for the assembly of multi-component innate immune complexes", Journal of Biological Chemistry 280 (2005): 32578-32585, DOI:10.1074/jbc.M503510200, https://hdl.handle.net/1912/2811
     
    All Items in WHOAS are protected by original copyright, with all rights reserved, unless otherwise indicated. WHOAS also supports the use of the Creative Commons licenses for original content.
    A service of the MBLWHOI Library | About WHOAS
    Contact Us | Send Feedback | Privacy Policy