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dc.contributor.authorZhang, Irene H.  Concept link
dc.contributor.authorMullen, Susan  Concept link
dc.contributor.authorCiccarese, Davide  Concept link
dc.contributor.authorDumit, Diana  Concept link
dc.contributor.authorMartocello, Donald E., III  Concept link
dc.contributor.authorToyofuku, Masanori  Concept link
dc.contributor.authorNomura, Nobuhiko  Concept link
dc.contributor.authorSmriga, Steven  Concept link
dc.contributor.authorBabbin, Andrew R.  Concept link
dc.date.accessioned2021-11-23T21:44:30Z
dc.date.available2021-11-23T21:44:30Z
dc.date.issued2021-09-10
dc.identifier.citationZhang, I. H., Mullen, S., Ciccarese, D., Dumit, D., Martocello, D. E., Toyofuku, M., Nomura, N., Smriga, S., & Babbin, A. R. (2021). Ratio of electron donor to acceptor influences metabolic specialization and denitrification dynamics in Pseudomonas aeruginosa in a mixed carbon medium. Frontiers in Microbiology, 12, 711073.en_US
dc.identifier.urihttps://hdl.handle.net/1912/27781
dc.description© The Author(s), 2021. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Zhang, I. H., Mullen, S., Ciccarese, D., Dumit, D., Martocello, D. E., Toyofuku, M., Nomura, N., Smriga, S., & Babbin, A. R. Ratio of electron donor to acceptor influences metabolic specialization and denitrification dynamics in Pseudomonas aeruginosa in a mixed carbon medium. Frontiers in Microbiology, 12, (2021): 711073, https://doi.org/10.3389/fmicb.2021.711073.en_US
dc.description.abstractDenitrifying microbes sequentially reduce nitrate (NO3–) to nitrite (NO2–), NO, N2O, and N2 through enzymes encoded by nar, nir, nor, and nos. Some denitrifiers maintain the whole four-gene pathway, but others possess partial pathways. Partial denitrifiers may evolve through metabolic specialization whereas complete denitrifiers may adapt toward greater metabolic flexibility in nitrogen oxide (NOx–) utilization. Both exist within natural environments, but we lack an understanding of selective pressures driving the evolution toward each lifestyle. Here we investigate differences in growth rate, growth yield, denitrification dynamics, and the extent of intermediate metabolite accumulation under varying nutrient conditions between the model complete denitrifier Pseudomonas aeruginosa and a community of engineered specialists with deletions in the denitrification genes nar or nir. Our results in a mixed carbon medium indicate a growth rate vs. yield tradeoff between complete and partial denitrifiers, which varies with total nutrient availability and ratios of organic carbon to NOx–. We found that the cultures of both complete and partial denitrifiers accumulated nitrite and that the metabolic lifestyle coupled with nutrient conditions are responsible for the extent of nitrite accumulation.en_US
dc.description.sponsorshipFunding for this work was provided by Simons Foundation award 622065 and an MIT Environmental Solutions Initiative seed grant to AB. Additional support was received by the MIT Ferry Fund.en_US
dc.publisherFrontiers Mediaen_US
dc.relation.urihttps://doi.org/10.3389/fmicb.2021.711073
dc.rightsAttribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectPseudomonas aeruginosaen_US
dc.subjectdenitrificationen_US
dc.subjectrate-yield tradeoffen_US
dc.subjectspecializationen_US
dc.subjectnitriteen_US
dc.titleRatio of electron donor to acceptor influences metabolic specialization and denitrification dynamics in Pseudomonas aeruginosa in a mixed carbon mediumen_US
dc.typeArticleen_US
dc.identifier.doi10.3389/fmicb.2021.711073


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Attribution 4.0 International
Except where otherwise noted, this item's license is described as Attribution 4.0 International