Real-time polarization microscopy of fibrillar collagen in histopathology

View/ Open
Date
2021-09-24Author
Keikhosravi, Adib
Concept link
Shribak, Michael
Concept link
Conklin, Matthew W.
Concept link
Liu, Yuming
Concept link
Li, Bin
Concept link
Loeffler, Agnes
Concept link
Levenson, Richard
Concept link
Eliceiri, Kevin
Concept link
Metadata
Show full item recordCitable URI
https://hdl.handle.net/1912/27775As published
https://doi.org/10.1038/s41598-021-98600-wDOI
10.1038/s41598-021-98600-wAbstract
Over the past two decades, fibrillar collagen reorganization parameters such as the amount of collagen deposition, fiber angle and alignment have been widely explored in numerous studies. These parameters are now widely accepted as stromal biomarkers and linked to disease progression and survival time in several cancer types. Despite all these advances, there has not been a significant effort to make it possible for clinicians to explore these biomarkers without adding steps to the clinical workflow or by requiring high-cost imaging systems. In this paper, we evaluate previously described polychromatic polarization microscope (PPM) to visualize collagen fibers with an optically generated color representation of fiber orientation and alignment when inspecting the sample by a regular microscope with minor modifications. This system does not require stained slides, but is compatible with histological stains such as H&E. Consequently, it can be easily accommodated as part of regular pathology review of tissue slides, while providing clinically useful insight into stromal composition.
Description
© The Author(s), 2021. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Keikhosravi, A., Shribak, M., Conklin, M. W., Liu, Y., Li, B., Loeffler, A., Levenson, R. M., & Eliceiri, K. W. Real-time polarization microscopy of fibrillar collagen in histopathology. Scientific Reports, 11(1), (2021): 19063, https://doi.org/10.1038/s41598-021-98600-w.
Collections
Suggested Citation
Keikhosravi, A., Shribak, M., Conklin, M. W., Liu, Y., Li, B., Loeffler, A., Levenson, R. M., & Eliceiri, K. W. (2021). Real-time polarization microscopy of fibrillar collagen in histopathology. Scientific Reports, 11(1), 19063.The following license files are associated with this item: