A phosphate starvation response gene (psr1-like) is present and expressed in Micromonas pusilla and other marine algae
Date
2021-02-25Author
Fiore, Cara L.
Concept link
Alexander, Harriet
Concept link
Kido Soule, Melissa C.
Concept link
Kujawinski, Elizabeth B.
Concept link
Metadata
Show full item recordCitable URI
https://hdl.handle.net/1912/27564As published
https://doi.org/10.3354/ame01955DOI
10.3354/ame01955Keyword
Micromonas pusilla; Phosphate stress response; Marine algae; Metabolomics; Dissolved organic matter; Biological oceanographyAbstract
Phosphorus (P) limits primary production in regions of the surface ocean, and many plankton species exhibit specific physiological responses to P deficiency. The metabolic response of Micromonas pusilla, an ecologically relevant marine photoautotroph, to P deficiency was investigated using metabolomics and comparative genomics. The concentrations of some intracellular metabolites were elevated in the P-deficient cells (e.g. xanthine, inosine), and genes involved in the associated metabolic pathways shared a predicted conserved amino acid motif in the non-coding regions of each gene. The presence of the conserved motif suggests that these genes may be co-regulated, and the motif may constitute a regulatory element for binding a transcription factor, specifically that of Psr1 (phosphate starvation response). A putative phosphate starvation response gene ( psr1-like) was identified in M. pusilla with homology to well characterized psr1/ phr1 genes in algae and plants, respectively. This gene appears to be present and expressed in other marine algal taxa (e.g. Emiliania huxleyi) in field sites that are chronically P limited. Results from the present study have implications for understanding phytoplankton taxon-specific roles in mediating P cycling in the ocean.
Description
© The Author(s), 2021. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Fiore, C. L., Alexander, H., Soule, M. C. K., & Kujawinski, E. B. A phosphate starvation response gene (psr1-like) is present and expressed in Micromonas pusilla and other marine algae. Aquatic Microbial Ecology, 86, (2021): 29–46, https://doi.org/10.3354/ame01955.
Collections
Suggested Citation
Fiore, C. L., Alexander, H., Soule, M. C. K., & Kujawinski, E. B. (2021). A phosphate starvation response gene (psr1-like) is present and expressed in Micromonas pusilla and other marine algae. Aquatic Microbial Ecology, 86, 29–46.The following license files are associated with this item:
Related items
Showing items related by title, author, creator and subject.
-
Proteomic responses of oceanic Synechococcus WH8102 to phosphate and zinc scarcity and cadmium additions
Cox, Alysia D.; Saito, Mak A. (Frontiers Media, 2013-12-17)Synechococcus sp. WH 8102 is a motile marine cyanobacterium isolated originally from the Sargasso Sea. To test the response of this organism to cadmium (Cd), generally considered a toxin, cultures were grown in a matrix ... -
Differential cellular responses associated with oxidative stress and cell fate decision under nitrate and phosphate limitations in Thalassiosira pseudonana : comparative proteomics
Lin, Qun; Liang, Jun-Rong; Huang, Qian-Qian; Luo, Chun-Shan; Anderson, Donald M.; Bowler, Chris; Chen, Chang-Ping; Li, Xue-Song; Gao, Ya-Hui (Public Library of Science, 2017-09-14)Diatoms are important components of marine ecosystems and contribute greatly to the world's primary production. Despite their important roles in ecosystems, the molecular basis of how diatoms cope with oxidative stress ... -
Evaluation of mixotrophy-associated gene expression in two species of polar marine algae
McKie-Krisberg, Zaid M.; Sanders, Robert W.; Gast, Rebecca J. (Frontiers Media, 2018-08-14)Mixotrophic flagellates can comprise significant proportions of plankton biomass in marine ecosystems. Despite the growing recognition of the importance of this ecological strategy, and the identification of major environmental ...