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dc.contributor.authorPérez Castro, Sherlynette  Concept link
dc.contributor.authorBorton, Mikayla A.  Concept link
dc.contributor.authorRegan, Kathleen M.  Concept link
dc.contributor.authorHrabe de Angelis, Isabella  Concept link
dc.contributor.authorWrighton, Kelly C.  Concept link
dc.contributor.authorTeske, Andreas P.  Concept link
dc.contributor.authorStrous, Marc  Concept link
dc.contributor.authorRuff, S. Emil  Concept link
dc.date.accessioned2021-08-31T19:16:48Z
dc.date.available2021-08-31T19:16:48Z
dc.date.issued2021-06-10
dc.identifier.citationCastro, S. P., Borton, M. A., Regan, K., de Angelis, I. H., Wrighton, K. C., Teske, A. P., Strous, M., & Ruff, S. E. (2021). Degradation of biological macromolecules supports uncultured microbial populations in Guaymas Basin hydrothermal sediments. Isme Journal.en_US
dc.identifier.urihttps://hdl.handle.net/1912/27516
dc.description© The Author(s), 2021. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Castro, S. P., Borton, M. A., Regan, K., de Angelis, I. H., Wrighton, K. C., Teske, A. P., Strous, M., & Ruff, S. E. Degradation of biological macromolecules supports uncultured microbial populations in Guaymas Basin hydrothermal sediments. Isme Journal. (2021), https://doi.org/10.1038/s41396-021-01026-5.en_US
dc.description.abstractHydrothermal sediments contain large numbers of uncultured heterotrophic microbial lineages. Here, we amended Guaymas Basin sediments with proteins, polysaccharides, nucleic acids or lipids under different redox conditions and cultivated heterotrophic thermophiles with the genomic potential for macromolecule degradation. We reconstructed 20 metagenome-assembled genomes (MAGs) of uncultured lineages affiliating with known archaeal and bacterial phyla, including endospore-forming Bacilli and candidate phylum Marinisomatota. One Marinisomatota MAG had 35 different glycoside hydrolases often in multiple copies, seven extracellular CAZymes, six polysaccharide lyases, and multiple sugar transporters. This population has the potential to degrade a broad spectrum of polysaccharides including chitin, cellulose, pectin, alginate, chondroitin, and carrageenan. We also describe thermophiles affiliating with the genera Thermosyntropha, Thermovirga, and Kosmotoga with the capability to make a living on nucleic acids, lipids, or multiple macromolecule classes, respectively. Several populations seemed to lack extracellular enzyme machinery and thus likely scavenged oligo- or monomers (e.g., MAGs affiliating with Archaeoglobus) or metabolic products like hydrogen (e.g., MAGs affiliating with Thermodesulfobacterium or Desulforudaceae). The growth of methanogens or the production of methane was not observed in any condition, indicating that the tested macromolecules are not degraded into substrates for methanogenesis in hydrothermal sediments. We provide new insights into the niches, and genomes of microorganisms that actively degrade abundant necromass macromolecules under oxic, sulfate-reducing, and fermentative thermophilic conditions. These findings improve our understanding of the carbon flow across trophic levels and indicate how primary produced biomass sustains complex and productive ecosystems.en_US
dc.description.sponsorshipWe are grateful to the captain and crew of the R/V Atlantis AT37-06 as well as the crew of the human occupied vehicle Alvin for their tireless support. Sampling at Guaymas Basin was supported by NSF (OCE-1357238).en_US
dc.publisherSpringer Natureen_US
dc.relation.urihttps://doi.org/10.1038/s41396-021-01026-5
dc.rightsAttribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.titleDegradation of biological macromolecules supports uncultured microbial populations in Guaymas Basin hydrothermal sedimentsen_US
dc.typeArticleen_US
dc.identifier.doi10.1038/s41396-021-01026-5


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Attribution 4.0 International
Except where otherwise noted, this item's license is described as Attribution 4.0 International