Discrete raw measurements from upwelling experiments conducted on two phytoplankton species isolated from the California Upwelling Zone

Abstract

Two phytoplankton species, Chaetoceros decipiens (UNC1416) and Emiliania huxleyi (UNC1419), were cultured at 12 °C in artificial Aquil* medium using trace metal clean (TMC) techniques. Macronutrients were supplied such that nitrate would become limiting for growth (50 μmol L⁻¹ NO3, 10 μmol L⁻¹ PO4, 200 μmol L⁻¹ H4SiO4) and two iron treatments were used (1370 nmol L⁻¹ and 3.1 nmol L⁻¹ total Fe). Upwelling conveyor belt cycle (UCBC) simulations were performed by transitioning the cultures to different nutrient and light regimes. They were grown by first growing the cultures with ample light (115 µmol photons m⁻² s⁻¹) until nitrate depletion and stationary growth. Cultures were then moved to no light for 10 days to simulate the sinking out of the euphotic zone. After this dark period, the cultures were subsequently transferred back to fresh medium under ambient light levels, and grown until stationary phase again. Samples were collected for six different time points associated with the different phases throughout the simulated upwelling cycle. This dataset includes the treatment information, replicate IDs, and the discrete raw measurements made for the upwelling experiments.

For a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/826448