Affinity purification of label-free tubulins from xenopus egg extracts
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https://hdl.handle.net/1912/26818As published
https://doi.org/10.1016/j.xpro.2020.100151DOI
10.1016/j.xpro.2020.100151Abstract
Cytoplasmic extracts from unfertilized Xenopus eggs have made important contributions to our understanding of microtubule dynamics, spindle assembly, and scaling. Until recently, these in vitro studies relied on the use of heterologous tubulin. This protocol allows for the purification of physiologically relevant Xenopus tubulins in milligram yield, which are a complex mixture of isoforms with various post-translational modifications. The protocol is applicable to any cell or tissue of interest.
For complete details on the use and execution of this protocol, please refer to Hirst et al. (2020).
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© The Author(s), 2020. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Reusch, S., Biswas, A., Hirst, W. G., & Reber, S. Affinity purification of label-free tubulins from xenopus egg extracts. STAR Protocols, 1(3), (2020): 100151, doi:10.1016/j.xpro.2020.100151.
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Suggested Citation
Reusch, S., Biswas, A., Hirst, W. G., & Reber, S. (2020). Affinity purification of label-free tubulins from xenopus egg extracts. STAR Protocols, 1(3), 100151.The following license files are associated with this item:
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivatives 4.0 International