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    Proteomic identification, cDNA cloning and enzymatic activity of glutathione S-transferases from the generalist marine gastropod, Cyphoma gibbosum

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    Whalen_ABB_accepted.pdf (3.309Mb)
    Date
    2008-06
    Author
    Whalen, Kristen E.  Concept link
    Morin, Dexter  Concept link
    Lin, Ching Yu  Concept link
    Tjeerdema, Ronald S.  Concept link
    Goldstone, Jared V.  Concept link
    Hahn, Mark E.  Concept link
    Metadata
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    Citable URI
    https://hdl.handle.net/1912/2594
    As published
    https://doi.org/10.1016/j.abb.2008.07.007
    Keyword
     Cyphoma gibbosum; Glutathione S-transferase; Gorgonian; Natural product; Chemical ecology; Allelochemical; Proteomic 
    Abstract
    Glutathione S-transferases (GST) were characterized from the digestive gland of Cyphoma gibbosum (Mollusca; Gastropoda), to investigate the possible role of these detoxification enzymes in conferring resistance to allelochemicals present in its gorgonian coral diet. We identified the collection of expressed cytosolic Cyphoma GST classes using a proteomic approach involving affinity chromatography, HPLC and nanospray liquid chromatography-tandem mass spectrometry (LC-MS/MS). Two major GST subunits were identified as putative mu-class GSTs; while one minor GST subunit was identified as a putative theta-class GST, apparently the first theta-class GST identified from a mollusc. Two Cyphoma GST cDNAs (CgGSTM1 and CgGSTM2) were isolated by RT-PCR using primers derived from peptide sequences. Phylogenetic analyses established both cDNAs as mu-class GSTs and revealed a mollusc-specific subclass of the GST-mu clade. These results provide new insights into metazoan GST diversity and the biochemical mechanisms used by marine organisms to cope with their chemically defended prey.
    Description
    Author Posting. © Elsevier B.V., 2008. This is the author's version of the work. It is posted here by permission of Elsevier B.V. for personal use, not for redistribution. The definitive version was published in Archives of Biochemistry and Biophysics 478 (2008): 7-17, doi:10.1016/j.abb.2008.07.007.
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    • Biology
    Suggested Citation
    Preprint: Whalen, Kristen E., Morin, Dexter, Lin, Ching Yu, Tjeerdema, Ronald S., Goldstone, Jared V., Hahn, Mark E., "Proteomic identification, cDNA cloning and enzymatic activity of glutathione S-transferases from the generalist marine gastropod, Cyphoma gibbosum", 2008-06, https://doi.org/10.1016/j.abb.2008.07.007, https://hdl.handle.net/1912/2594
     

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