Proteomic identification, cDNA cloning and enzymatic activity of glutathione S-transferases from the generalist marine gastropod, Cyphoma gibbosum

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2008-06Author
Whalen, Kristen E.
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Morin, Dexter
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Lin, Ching Yu
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Tjeerdema, Ronald S.
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Goldstone, Jared V.
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Hahn, Mark E.
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https://hdl.handle.net/1912/2594As published
https://doi.org/10.1016/j.abb.2008.07.007Keyword
Cyphoma gibbosum; Glutathione S-transferase; Gorgonian; Natural product; Chemical ecology; Allelochemical; ProteomicAbstract
Glutathione S-transferases (GST) were characterized from the digestive gland of
Cyphoma gibbosum (Mollusca; Gastropoda), to investigate the possible role of these
detoxification enzymes in conferring resistance to allelochemicals present in its gorgonian coral
diet. We identified the collection of expressed cytosolic Cyphoma GST classes using a
proteomic approach involving affinity chromatography, HPLC and nanospray liquid
chromatography-tandem mass spectrometry (LC-MS/MS). Two major GST subunits were
identified as putative mu-class GSTs; while one minor GST subunit was identified as a putative
theta-class GST, apparently the first theta-class GST identified from a mollusc. Two Cyphoma
GST cDNAs (CgGSTM1 and CgGSTM2) were isolated by RT-PCR using primers derived from
peptide sequences. Phylogenetic analyses established both cDNAs as mu-class GSTs and
revealed a mollusc-specific subclass of the GST-mu clade. These results provide new insights
into metazoan GST diversity and the biochemical mechanisms used by marine organisms to cope
with their chemically defended prey.
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Author Posting. © Elsevier B.V., 2008. This is the author's version of the work. It is posted here by permission of Elsevier B.V. for personal use, not for redistribution. The definitive version was published in Archives of Biochemistry and Biophysics 478 (2008): 7-17, doi:10.1016/j.abb.2008.07.007.
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Preprint: Whalen, Kristen E., Morin, Dexter, Lin, Ching Yu, Tjeerdema, Ronald S., Goldstone, Jared V., Hahn, Mark E., "Proteomic identification, cDNA cloning and enzymatic activity of glutathione S-transferases from the generalist marine gastropod, Cyphoma gibbosum", 2008-06, https://doi.org/10.1016/j.abb.2008.07.007, https://hdl.handle.net/1912/2594Related items
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