Extra centrosomes and/or chromosomes prolong mitosis in human cells
Rieder, Conly L.
MetadataShow full item record
Using laser microsurgery and cell fusion we have explored how additional centrosomes and/or chromosomes influence the duration of mitosis in human cells. We find that doubling the chromosome number adds ~10 minutes to a 20 minute division while doubling the number of centrosomes adds ~30 minutes more, and extra centrosomes and/or chromosomes prolong mitosis by delaying satisfaction of the spindle assembly checkpoint. Thus mitosis can be prolonged by non genetic means and extra chromosomes and centrosomes likely contribute to the elevated mitotic index seen in many tumors.
Author Posting. © The Author(s), 2008. This is the author's version of the work. It is posted here by permission of Nature Publishing Group for personal use, not for redistribution. The definitive version was published in Nature Cell Biology 10 (2008): 748-751, doi:10.1038/ncb1738.
Showing items related by title, author, creator and subject.
Goshima, Gohta; Wollman, Roy; Stuurman, Nico; Scholey, Jonathan M.; Vale, Ronald D. (2005-09-30)The pole-to-pole distance of the metaphase spindle is reasonably constant in a given cell type; in the case of vertebrate female oocytes, this steady-state length can be maintained for substantial lengths of time, during ...
Inoue, Shinya (2008-04-11)This Perspective is an account of my early experience while I studied the dynamic organization and behavior of the mitotic spindle and its submicroscopic filaments using polarized light microscopy. The birefringence of ...
Rate zonal density gradient ultracentrifugation analysis of repair of radiation damage to the folded chromosome of Escherichia coli Ulmer, Kevin M. (Massachusetts Institute of Technology and Woods Hole Oceanographic Institution, 1978-04)The structure of the membrane-free nucleoid of Escherichia coli and of unfolded chromosomal DNA was investigated by sedimentation on neutral sucrose gradients after irradiation with 60Co gamma-rays and ultraviolet light ...