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dc.contributor.authorKeya, Jakia Jannat  Concept link
dc.contributor.authorKudoh, Hiroki  Concept link
dc.contributor.authorKabir, Arif Md. Rashedul  Concept link
dc.contributor.authorInoue, Daisuke  Concept link
dc.contributor.authorMiyamoto, Nobuyoshi  Concept link
dc.contributor.authorTani, Tomomi  Concept link
dc.contributor.authorKakugo, Akira  Concept link
dc.contributor.authorShikinaka, Kazuhiro  Concept link
dc.date.accessioned2020-04-29T19:06:32Z
dc.date.available2020-04-29T19:06:32Z
dc.date.issued2020-04-10
dc.identifier.citationKeya, J. J., Kudoh, H., Kabir, A. M. R., Inoue, D., Miyamoto, N., Tani, T., Kakugo, A., & Shikinaka, K. (2020). Radial alignment of microtubules through tubulin polymerization in an evaporating droplet. Plos One, 15(4), e0231352.en_US
dc.identifier.urihttps://hdl.handle.net/1912/25710
dc.description© The Author(s), 2020. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Keya, J. J., Kudoh, H., Kabir, A. M. R., Inoue, D., Miyamoto, N., Tani, T., Kakugo, A., & Shikinaka, K. Radial alignment of microtubules through tubulin polymerization in an evaporating droplet. Plos One, 15(4), (2020): e0231352, doi:10.1371/journal.pone.0231352.en_US
dc.description.abstractWe report the formation of spherulites from droplets of highly concentrated tubulin solution via nucleation and subsequent polymerization to microtubules (MTs) under water evaporation by heating. Radial alignment of MTs in the spherulites was confirmed by the optical properties of the spherulites observed using polarized optical microscopy and fluorescence microscopy. Temperature and concentration of tubulins were found as important parameters to control the spherulite pattern formation of MTs where evaporation plays a significant role. The alignment of MTs was regulated reversibly by temperature induced polymerization and depolymerization of tubulins. The formation of the MTs patterns was also confirmed at the molecular level from the small angle X-ray measurements. This work provides a simple method for obtaining radially aligned arrays of MTs.en_US
dc.description.sponsorshipFund receiver: Akira Kakugo Grant-in-Aid for Scientific Research on Innovative Areas (Grant Nos. JP24104004 and 18H05423) and a Grant-in-Aid for Scientific Research (A) (Grant No. 18H03673) from kaken. NO - The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscripten_US
dc.publisherPublic Library of Scienceen_US
dc.relation.urihttps://doi.org/10.1371/journal.pone.0231352
dc.rightsAttribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.titleRadial alignment of microtubules through tubulin polymerization in an evaporating dropleten_US
dc.typeArticleen_US
dc.identifier.doi10.1371/journal.pone.0231352


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Attribution 4.0 International
Except where otherwise noted, this item's license is described as Attribution 4.0 International