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dc.contributor.authorKuloyo, Olukayode  Concept link
dc.contributor.authorRuff, S. Emil  Concept link
dc.contributor.authorCahill, Aaron  Concept link
dc.contributor.authorConnors, Liam  Concept link
dc.contributor.authorZorz, Jackie K.  Concept link
dc.contributor.authorHrabe de Angelis, Isabella  Concept link
dc.contributor.authorNightingale, Michael  Concept link
dc.contributor.authorMayer, Bernhard  Concept link
dc.contributor.authorStrous, Marc  Concept link
dc.date.accessioned2020-03-17T18:40:27Z
dc.date.available2020-03-17T18:40:27Z
dc.date.issued2020-02-03
dc.identifier.citationKuloyo, O., Ruff, S. E., Cahill, A., Connors, L., Zorz, J. K., de Angelis, I. H., Nightingale, M., Mayer, B., & Strous, M. (2020). Methane oxidation and methylotroph population dynamics in groundwater mesocosms. Environmental Microbiology.en_US
dc.identifier.urihttps://hdl.handle.net/1912/25537
dc.description© The Author(s), 2020. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Kuloyo, O., Ruff, S. E., Cahill, A., Connors, L., Zorz, J. K., de Angelis, I. H., Nightingale, M., Mayer, B., & Strous, M. Methane oxidation and methylotroph population dynamics in groundwater mesocosms. Environmental Microbiology. (2020), doi:10.1111/1462-2920.14929.en_US
dc.description.abstractExtraction of natural gas from unconventional hydrocarbon reservoirs by hydraulic fracturing raises concerns about methane migration into groundwater. Microbial methane oxidation can be a significant methane sink. Here, we inoculated replicated, sand‐packed, continuous mesocosms with groundwater from a field methane release experiment. The mesocosms experienced thirty‐five weeks of dynamic methane, oxygen and nitrate concentrations. We determined concentrations and stable isotope signatures of methane, carbon dioxide and nitrate and monitored microbial community composition of suspended and attached biomass. Methane oxidation was strictly dependent on oxygen availability and led to enrichment of 13C in residual methane. Nitrate did not enhance methane oxidation under oxygen limitation. Methylotrophs persisted for weeks in the absence of methane, making them a powerful marker for active as well as past methane leaks. Thirty‐nine distinct populations of methylotrophic bacteria were observed. Methylotrophs mainly occurred attached to sediment particles. Abundances of methanotrophs and other methylotrophs were roughly similar across all samples, pointing at transfer of metabolites from the former to the latter. Two populations of Gracilibacteria (Candidate Phyla Radiation) displayed successive blooms, potentially triggered by a period of methane famine. This study will guide interpretation of future field studies and provides increased understanding of methylotroph ecophysiology.en_US
dc.description.sponsorshipThe authors acknowledge funding from the Alberta Innovates Technology Futures (AITF), and University of Calgary Eyes High Doctoral Scholarships (O.O.K., J.K.Z.) and AITF/Eyes High Postdoctoral Fellowships (S.E.R.), as well as the PROMOS Internship Abroad Scholarship by the German Academic Exchange Service (I.H.d.A.). Additional support was provided by the Natural Sciences and Engineering Research Council of Canada (NSERC), Strategic Project Grant no. 463045‐14, the Campus Alberta Innovation Chair Program (M.S.), Alberta Innovates, The Canadian Foundation for Innovation (M.S.), the Alberta Small Equipment Grant Program (M.S.) and an NSERC Discovery Grant (M.S. and B.M.).en_US
dc.publisherWileyen_US
dc.relation.urihttps://doi.org/10.1111/1462-2920.14929
dc.rightsAttribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.titleMethane oxidation and methylotroph population dynamics in groundwater mesocosmsen_US
dc.typeArticleen_US
dc.identifier.doi10.1111/1462-2920.14929


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Attribution 4.0 International
Except where otherwise noted, this item's license is described as Attribution 4.0 International