Show simple item record

dc.contributor.authorInomura, Keisuke  Concept link
dc.contributor.authorDeutsch, Curtis A.  Concept link
dc.contributor.authorWilson, Samuel T.  Concept link
dc.contributor.authorMasuda, Takako  Concept link
dc.contributor.authorLawrenz, Evelyn  Concept link
dc.contributor.authorBučinská, Lenka  Concept link
dc.contributor.authorSobotka, Roman  Concept link
dc.contributor.authorGauglitz, Julia M.  Concept link
dc.contributor.authorSaito, Mak A.  Concept link
dc.contributor.authorPrášil, Ondřej  Concept link
dc.contributor.authorFollows, Michael J.  Concept link
dc.date.accessioned2020-02-26T16:26:35Z
dc.date.available2020-02-26T16:26:35Z
dc.date.issued2019-12-11
dc.identifier.citationInomura, K., Deutsch, C., Wilson, S. T., Masuda, T., Lawrenz, E., Lenka, B., Sobotka, R., Gauglitz, J. M., Saito, M. A., Prášil, O., & Follows, M. J. (2019). Quantifying oxygen management and temperature and light dependencies of nitrogen fixation by Crocosphaera watsonii. Msphere, 4(6), e00531-19.en_US
dc.identifier.urihttps://hdl.handle.net/1912/25437
dc.description© The Author(s), 2019. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Inomura, K., Deutsch, C., Wilson, S. T., Masuda, T., Lawrenz, E., Lenka, B., Sobotka, R., Gauglitz, J. M., Saito, M. A., Prášil, O., & Follows, M. J. Quantifying oxygen management and temperature and light dependencies of nitrogen fixation by Crocosphaera watsonii. Msphere, 4(6), (2019): e00531-19, doi: 10.1128/msphere.00531-19.en_US
dc.description.abstractCrocosphaera is a major dinitrogen (N2)-fixing microorganism, providing bioavailable nitrogen (N) to marine ecosystems. The N2-fixing enzyme nitrogenase is deactivated by oxygen (O2), which is abundant in marine environments. Using a cellular scale model of Crocosphaera sp. and laboratory data, we quantify the role of three O2 management strategies by Crocosphaera sp.: size adjustment, reduced O2 diffusivity, and respiratory protection. Our model predicts that Crocosphaera cells increase their size under high O2. Using transmission electron microscopy, we show that starch granules and thylakoid membranes are located near the cytoplasmic membranes, forming a barrier for O2. The model indicates a critical role for respiration in protecting the rate of N2 fixation. Moreover, the rise in respiration rates and the decline in ambient O2 with temperature strengthen this mechanism in warmer water, providing a physiological rationale for the observed niche of Crocosphaera at temperatures exceeding 20°C. Our new measurements of the sensitivity to light intensity show that the rate of N2 fixation reaches saturation at a lower light intensity (∼100 μmol m−2 s−1) than photosynthesis and that both are similarly inhibited by light intensities of >500 μmol m−2 s−1. This suggests an explanation for the maximum population of Crocosphaera occurring slightly below the ocean surface.en_US
dc.description.sponsorshipWe thank Stephanie Dutkiewicz and Sallie W. Chisholm for useful discussion, Martin Lukeš for technical assistance for the N2 fixation measurement, and the members of Writing and Communication Center at MIT for their advice on writing. This research was supported by the Japan Student Service Organization (JASSO) (grant L11171020001 to K.I.), the Gordon and Betty Moore Foundation (grant GBMF 3775 to C.D. and grant GBMF 3778 to M.J.F.), the U.S. National Science Foundation (grant OCE-1756524 to S.T.W., grant OCE-1558702 to M.J.F., and grant OCE-PRF 1421196 to J.M.G), the Simons Foundation (Simons Postdoctoral Fellowship in Marine Microbial Ecology award 544338 to K.I., Simons Collaboration on Ocean Processes and Ecology award 329108 to M.J.F., Simons Collaboration on Computational BIOgeochemical Modeling of Marine EcosystemS [CBIOMES] award 549931 to M.J.F.), the Czech Science Foundation (GAČR) (grant 16-15467S to O.P.), and the National Sustainability Programme (NPU) (grant LO1416 Algatech plus to O.P.).en_US
dc.publisherAmerican Society for Microbiologyen_US
dc.relation.urihttps://doi.org/10.1128/msphere.00531-19
dc.rightsAttribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectCrocosphaeraen_US
dc.subjectCarbonen_US
dc.subjectCell flux modelen_US
dc.subjectDaily cycleen_US
dc.subjectIronen_US
dc.subjectLighten_US
dc.subjectNitrogenen_US
dc.subjectNitrogen fixationen_US
dc.subjectOxygenen_US
dc.subjectPhotosynthesisen_US
dc.subjectTemperatureen_US
dc.titleQuantifying oxygen management and temperature and light dependencies of nitrogen fixation by Crocosphaera watsoniien_US
dc.typeArticleen_US
dc.identifier.doi10.1128/msphere.00531-19


Files in this item

Thumbnail
Thumbnail
Thumbnail
Thumbnail
Thumbnail
Thumbnail
Thumbnail

This item appears in the following Collection(s)

Show simple item record

Attribution 4.0 International
Except where otherwise noted, this item's license is described as Attribution 4.0 International