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dc.contributor.authorWilliams, Laura E.  Concept link
dc.contributor.authorCullen, Nicole  Concept link
dc.contributor.authorDeGiorgis, Joseph A.  Concept link
dc.contributor.authorMartinez, Karla J.  Concept link
dc.contributor.authorMellone, Justina  Concept link
dc.contributor.authorOser, Molly  Concept link
dc.contributor.authorWang, Jing  Concept link
dc.contributor.authorZhang, Ying  Concept link
dc.date.accessioned2020-01-31T20:10:57Z
dc.date.available2020-01-31T20:10:57Z
dc.date.issued2019-12-01
dc.identifier.citationWilliams, L. E., Cullen, N., DeGiorgis, J. A., Martinez, K. J., Mellone, J., Oser, M., Wang, J., & Zhang, Y. (2019). Variation in genome content and predatory phenotypes between Bdellovibrio sp. NC01 isolated from soil and B. bacteriovorus type strain HD100. Microbiology, Variation in genome content and predatory phenotypes between Bdellovibrio sp. NC01 isolated from soil and B. bacteriovorus type strain HD100. Microbiology, 165(12), 1315-1330.en_US
dc.identifier.urihttps://hdl.handle.net/1912/25297
dc.description© The Author(s), 2019. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Williams, L. E., Cullen, N., DeGiorgis, J. A., Martinez, K. J., Mellone, J., Oser, M., Wang, J., & Zhang, Y. Variation in genome content and predatory phenotypes between Bdellovibrio sp. NC01 isolated from soil and B. bacteriovorus type strain HD100. Microbiology, 165(12), (2019): 1315-1330, doi: 10.1099/mic.0.000861.en_US
dc.description.abstractDefining phenotypic and associated genotypic variation among Bdellovibrio may further our understanding of how this genus attacks and kills different Gram-negative bacteria. We isolated Bdellovibrio sp. NC01 from soil. Analysis of 16S rRNA gene sequences and average amino acid identity showed that NC01 belongs to a different species than the type species bacteriovorus. By clustering amino acid sequences from completely sequenced Bdellovibrio and comparing the resulting orthologue groups to a previously published analysis, we defined a ‘core genome’ of 778 protein-coding genes and identified four protein-coding genes that appeared to be missing only in NC01. To determine how horizontal gene transfer (HGT) may have impacted NC01 genome evolution, we performed genome-wide comparisons of Bdellovibrio nucleotide sequences, which indicated that eight NC01 genomic regions were likely acquired by HGT. To investigate how genome variation may impact predation, we compared protein-coding gene content between NC01 and the B. bacteriovorus type strain HD100, focusing on genes implicated as important in successful killing of prey. Of these, NC01 is missing ten genes that may play roles in lytic activity during predation. Compared to HD100, NC01 kills fewer tested prey strains and kills Escherichia coli ML35 less efficiently. NC01 causes a smaller log reduction in ML35, after which the prey population recovers and the NC01 population decreases. In addition, NC01 forms turbid plaques on lawns of E. coli ML35, in contrast to clear plaques formed by HD100. Linking phenotypic variation in interactions between Bdellovibrio and Gram-negative bacteria with underlying Bdellovibrio genome variation is valuable for understanding the ecological significance of predatory bacteria and evaluating their effectiveness in clinical applications.en_US
dc.description.sponsorshipThis research was supported by an Institutional Development Award (IDeA) from the National Institute of General Medical Sciences of the National Institutes of Health under grant no. P20GM103430 and by funding to LEW from Providence College. This material is based upon work conducted at a Rhode Island NSF EPSCoR research facility, the Genomics and Sequencing Center, supported in part by the National Science Foundation EPSCoR Cooperative Agreement #EPS-1004057. This material is based upon work supported in part by the National Science Foundation under EPSCoR Cooperative Agreement #OIA-1655221. The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.en_US
dc.publisherMicrobiology Societyen_US
dc.relation.urihttps://doi.org/10.1099/mic.0.000861
dc.rightsAttribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectpredatory bacteriaen_US
dc.subjectprey rangeen_US
dc.subjectpredation efficiencyen_US
dc.subjectplaque phenotypeen_US
dc.subjectlytic enzymesen_US
dc.titleVariation in genome content and predatory phenotypes between Bdellovibrio sp. NC01 isolated from soil and B. bacteriovorus type strain HD100en_US
dc.typeArticleen_US
dc.identifier.doi10.1099/mic.0.000861


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Except where otherwise noted, this item's license is described as Attribution 4.0 International