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dc.contributor.authorBreitbart, Mya  Concept link
dc.contributor.authorBuck, Kristen N.  Concept link
dc.contributor.authorBonnain, Chelsea  Concept link
dc.contributor.authorCaprara, Salvatore  Concept link
dc.coverage.temporal20180622 - 20190207 (UTC)
dc.date.accessioned2020-01-27T21:20:05Z
dc.date.available2020-01-27T21:20:05Z
dc.date.created2019-02-27
dc.date.issued2020-01-27
dc.identifier.urihttps://hdl.handle.net/1912/25280
dc.descriptionDataset: Fe-Labeling Expt: E.coli and phage T4 or T5
dc.description.abstractThis data was collected as part of a study investigating the source of iron to bacteriophage (phage for short, or viruses that infect and kill bacteria) progeny. Evidence from a phage that infects E. coli shows iron incorporated into the tail fiber structure. This study aims at identifying whether the source of the iron is environmental or bacterially derived. E. coli bacterial cultures were grown in minimal media spiked with 10 µM 57FeSO4 then infected with phage T4 or T5. The phages were purified by methods of centrifugation, filtration, density-dependent ultracentrifugation, and dialyzing. The resulting phage fractions were quantified by SYBR epifluorescence microscopy and metal concentrations were measured on an ELEMENT XR ICP-MS. For a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/757485
dc.description.sponsorshipNSF Division of Ocean Sciences (NSF OCE) OCE-1722761
dc.language.isoen_US
dc.publisherBiological and Chemical Oceanography Data Management Office (BCO-DMO). Contact: bco-dmo-data@whoi.edu
dc.relation.urihttp://lod.bco-dmo.org/id/dataset/757485
dc.relation.urihttps://doi.org/10.1575/1912/bco-dmo.757485.1
dc.rightsCreative Commons Attribution 4.0
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.titleIron concentrations of phage from experiments of iron-labelled E. coli infected with T4 and T5 bacteriophage, 2018 and 2019.
dc.typeDataset
dc.identifier.doi10.1575/1912/bco-dmo.757485.1


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Creative Commons Attribution 4.0
Except where otherwise noted, this item's license is described as Creative Commons Attribution 4.0