Show simple item record

dc.contributor.authorSt-Jean, Sylvie D.  Concept link
dc.contributor.authorStephens, Raymond E.  Concept link
dc.contributor.authorCourtenay, S. C.  Concept link
dc.contributor.authorReinisch, Carol L.  Concept link
dc.date.accessioned2005-12-13T17:07:32Z
dc.date.available2005-12-13T17:07:32Z
dc.date.issued2005-09-01
dc.identifier.citationCanadian Journal of Fisheries and Aquatic Sciences 62 (2005): 2055-2066en
dc.identifier.urihttps://hdl.handle.net/1912/251
dc.descriptionAuthor Posting. © National Research Council Canada, 2005. This article is posted here by permission of National Research Council Canada for personal use, not for redistribution. The definitive version was published in Canadian Journal of Fisheries and Aquatic Sciences 62 (2005): 2055-2066, doi:10.1139/F05-119.
dc.description.abstractEvaluating patterns of expression of p53-related proteins in cells is a novel approach in defining environmentally linked diseases. We have examined the induction of haemocytic leukemia in Mytilus edulis by municipal and industrial contaminants in Pictou Harbour, Nova Scotia, Canada. We used a murine monoclonal antibody, 1E10, as a diagnostic reagent to detect leukemic cells. We first characterized the reactivity of 1E10 with both normal and leukemic Mytilus haemocytes by confocal microscopy. We then compared p53 gene family expression (p53, p63–p73, and p97) in normal versus leukemic haemocytes using a panel of monoclonal and polyclonal antibodies to p53 family proteins. The immunochemical data demonstrate that haemocytic leukemia cells of M. edulis differentially express p63–p73 and p97–p120 proteins. We subsequently used 1E10 to diagnose haemocytic leukemia in 500 M. edulis previously deployed 6 months earlier in Pictou Harbour. In the field, Mytilus caged near untreated municipal wastewater and bleached kraft pulpmill effluents have a significantly greater chance of developing haemocytic leukemia than do mussels exposed to reference sites.en
dc.description.sponsorshipThis research was funded in part by the Pictou Harbour Biomonitoring Project, Fisheries and Oceans Canada, and Environment Canada.en
dc.format.extent1017761 bytes
dc.format.mimetypeapplication/pdf
dc.language.isoenen
dc.publisherNational Research Council Canadaen
dc.relation.urihttps://doi.org/10.1139/F05-119
dc.subjectEnvironmentally linked diseasesen
dc.subjectp53 proteinsen
dc.subjectMytilus edulisen
dc.titleDetecting p53 family proteins in haemocytic leukemia cells of Mytilus edulis from Pictou Harbour, Nova Scotia, Canadaen
dc.typeArticleen
dc.identifier.doi10.1139/F05-119


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record