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    Detecting p53 family proteins in haemocytic leukemia cells of Mytilus edulis from Pictou Harbour, Nova Scotia, Canada

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    St Jean Detecting.pdf (993.9Kb)
    Date
    2005-09-01
    Author
    St-Jean, Sylvie D.  Concept link
    Stephens, Raymond E.  Concept link
    Courtenay, S. C.  Concept link
    Reinisch, Carol L.  Concept link
    Metadata
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    Citable URI
    https://hdl.handle.net/1912/251
    As published
    https://doi.org/10.1139/F05-119
    DOI
    10.1139/F05-119
    Keyword
     Environmentally linked diseases; p53 proteins; Mytilus edulis 
    Abstract
    Evaluating patterns of expression of p53-related proteins in cells is a novel approach in defining environmentally linked diseases. We have examined the induction of haemocytic leukemia in Mytilus edulis by municipal and industrial contaminants in Pictou Harbour, Nova Scotia, Canada. We used a murine monoclonal antibody, 1E10, as a diagnostic reagent to detect leukemic cells. We first characterized the reactivity of 1E10 with both normal and leukemic Mytilus haemocytes by confocal microscopy. We then compared p53 gene family expression (p53, p63–p73, and p97) in normal versus leukemic haemocytes using a panel of monoclonal and polyclonal antibodies to p53 family proteins. The immunochemical data demonstrate that haemocytic leukemia cells of M. edulis differentially express p63–p73 and p97–p120 proteins. We subsequently used 1E10 to diagnose haemocytic leukemia in 500 M. edulis previously deployed 6 months earlier in Pictou Harbour. In the field, Mytilus caged near untreated municipal wastewater and bleached kraft pulpmill effluents have a significantly greater chance of developing haemocytic leukemia than do mussels exposed to reference sites.
    Description
    Author Posting. © National Research Council Canada, 2005. This article is posted here by permission of National Research Council Canada for personal use, not for redistribution. The definitive version was published in Canadian Journal of Fisheries and Aquatic Sciences 62 (2005): 2055-2066, doi:10.1139/F05-119.
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    • Laboratory of Aquatic Biomedicine
    Suggested Citation
    Canadian Journal of Fisheries and Aquatic Sciences 62 (2005): 2055-2066
     

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