Detecting p53 family proteins in haemocytic leukemia cells of Mytilus edulis from Pictou Harbour, Nova Scotia, Canada
St-Jean, Sylvie D.
Stephens, Raymond E.
Courtenay, S. C.
Reinisch, Carol L.
MetadataShow full item record
Evaluating patterns of expression of p53-related proteins in cells is a novel approach in defining environmentally linked diseases. We have examined the induction of haemocytic leukemia in Mytilus edulis by municipal and industrial contaminants in Pictou Harbour, Nova Scotia, Canada. We used a murine monoclonal antibody, 1E10, as a diagnostic reagent to detect leukemic cells. We first characterized the reactivity of 1E10 with both normal and leukemic Mytilus haemocytes by confocal microscopy. We then compared p53 gene family expression (p53, p63–p73, and p97) in normal versus leukemic haemocytes using a panel of monoclonal and polyclonal antibodies to p53 family proteins. The immunochemical data demonstrate that haemocytic leukemia cells of M. edulis differentially express p63–p73 and p97–p120 proteins. We subsequently used 1E10 to diagnose haemocytic leukemia in 500 M. edulis previously deployed 6 months earlier in Pictou Harbour. In the field, Mytilus caged near untreated municipal wastewater and bleached kraft pulpmill effluents have a significantly greater chance of developing haemocytic leukemia than do mussels exposed to reference sites.
Author Posting. © National Research Council Canada, 2005. This article is posted here by permission of National Research Council Canada for personal use, not for redistribution. The definitive version was published in Canadian Journal of Fisheries and Aquatic Sciences 62 (2005): 2055-2066, doi:10.1139/F05-119.
Showing items related by title, author, creator and subject.
Blythe, Jonathan N.; Lea, David W. (National Shellfisheries Association, 2008-04)A mussel's shell records its history of growth. We investigated variability in the size and shape of mussel shells of Mytilus californianus Conrad (1837) to test the hypothesis that the mussel shell provides information ...
Processing of ingested dimethylsulfoniopropionate by mussels Mytilus edulis and scallops Argopecten irradians Hill, Richard W.; Dacey, John W. H. (Inter-Research, 2007-08-07)Dimethylsulfoniopropionate (DMSP) synthesized by marine phytoplankton is the principal source of dimethylsulfide (DMS), an important climate-affecting gas. Grazing by small zooplankton on phytoplankton often accelerates ...
Exploring B/Ca as a pH proxy in bivalves : relationships between Mytilus californianus B/Ca and environmental data from the northeast Pacific McCoy, S. J.; Robinson, Laura F.; Pfister, Catherine A.; Wootton, J. T.; Shimizu, Nobumichi (Copernicus Publications on behalf of the European Geosciences Union, 2011-09-13)A distinct gap in our ability to understand changes in coastal biology that may be associated with recent ocean acidification is the paucity of directly measured ocean environmental parameters at coastal sites in recent ...