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    Foraminiferal ultrastructure: A perspective from fluorescent and fluorogenic probes

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    Article (2.894Mb)
    Supporting_Information_S1 (613.7Kb)
    Table_S1 (14.49Kb)
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    Date
    2019-08-22
    Author
    Frontalini, Fabrizio  Concept link
    Losada, Maria Teresa  Concept link
    Toyofuku, Takashi  Concept link
    Tyszka, Jarosław  Concept link
    Goleń, Jan  Concept link
    de Nooijer, Lennart  Concept link
    Canonico, Barbara  Concept link
    Cesarini, Erica  Concept link
    Nagai, Yukiko  Concept link
    Bickmeyer, Ulf  Concept link
    Ikuta, Tetsuro  Concept link
    Tsubaki, Remi  Concept link
    Rodriguez, Celia Besteiro  Concept link
    Al-Enezi, Eqbal  Concept link
    Papa, Stefano  Concept link
    Coccioni, Rodolfo  Concept link
    Bijma, Jelle  Concept link
    Bernhard, Joan M.  Concept link
    Metadata
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    Citable URI
    https://hdl.handle.net/1912/25088
    As published
    https://doi.org/10.1029/2019JG005113
    DOI
    10.1029/2019JG005113
    Keyword
     protist; organelles; confocal laser scanning microscopy; probes; foraminifera 
    Abstract
    Microscopy techniques have been widely applied to observe cellular ultrastructure. Most of these techniques, such as transmission electron microscopy, produce high‐resolution images, but they may require extensive preparation, hampering their application for in vivo examination. Other approaches, such as fluorescent and fluorogenic probes, can be applied not only to fixed specimens but also to living cells when the probes are nontoxic. Fluorescence‐based methods, which are generally relatively easy to use, allow visual and (semi)quantitative studies of the ultrastructural organization and processes of the cell under natural as well as manipulated conditions. To date, there are relatively few published studies on the nearly ubiquitous marine protistan group Foraminifera that have used fluorescent and fluorogenic probes, despite their huge potential. The aim of the present contribution is to document the feasible application of a wide array of these probes to foraminiferal biology. More specifically, we applied fluorescence‐based probes to study esterase activity, cell viability, calcium signaling, pH variation, reactive oxygen species, neutral and polar lipids, lipid droplets, cytoskeleton structures, Golgi complex, acidic vesicles, nuclei, and mitochondria in selected foraminiferal species.
    Description
    Author Posting. © American Geophysical Union, 2019. This article is posted here by permission of American Geophysical Union for personal use, not for redistribution. The definitive version was published in Journal of Geophysical Research-Biogeosciences 124 (2019): 2823-2850, doi:10.1029/2019JG005113.
    Collections
    • Geology and Geophysics (G&G)
    Suggested Citation
    Frontalini, F., Losada, M. T., Toyofuku, T., Tyszka, J., Golen, J., de Nooijer, L., Canonico, B., Cesarini, E., Nagai, Y., Bickmeyer, U., Ikuta, T., Tsubaki, R., Besteiro Rodriguez, C., Al Enezi, E., Papa, S., Coccioni, R., Bijma, J., & Bernhard, J. M. (2019). Foraminiferal ultrastructure: A perspective from fluorescent and fluorogenic probes. Journal of Geophysical Research-Biogeosciences, 124, 2823-2850.
     

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