Foraminiferal ultrastructure: A perspective from fluorescent and fluorogenic probes

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2019-08-22Author
Frontalini, Fabrizio
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Losada, Maria Teresa
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Toyofuku, Takashi
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Tyszka, Jarosław
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Goleń, Jan
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de Nooijer, Lennart
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Canonico, Barbara
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Cesarini, Erica
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Nagai, Yukiko
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Bickmeyer, Ulf
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Ikuta, Tetsuro
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Tsubaki, Remi
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Rodriguez, Celia Besteiro
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Al-Enezi, Eqbal
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Papa, Stefano
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Coccioni, Rodolfo
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Bijma, Jelle
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Bernhard, Joan M.
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https://hdl.handle.net/1912/25088As published
https://doi.org/10.1029/2019JG005113DOI
10.1029/2019JG005113Abstract
Microscopy techniques have been widely applied to observe cellular ultrastructure. Most of these techniques, such as transmission electron microscopy, produce high‐resolution images, but they may require extensive preparation, hampering their application for in vivo examination. Other approaches, such as fluorescent and fluorogenic probes, can be applied not only to fixed specimens but also to living cells when the probes are nontoxic. Fluorescence‐based methods, which are generally relatively easy to use, allow visual and (semi)quantitative studies of the ultrastructural organization and processes of the cell under natural as well as manipulated conditions. To date, there are relatively few published studies on the nearly ubiquitous marine protistan group Foraminifera that have used fluorescent and fluorogenic probes, despite their huge potential. The aim of the present contribution is to document the feasible application of a wide array of these probes to foraminiferal biology. More specifically, we applied fluorescence‐based probes to study esterase activity, cell viability, calcium signaling, pH variation, reactive oxygen species, neutral and polar lipids, lipid droplets, cytoskeleton structures, Golgi complex, acidic vesicles, nuclei, and mitochondria in selected foraminiferal species.
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Author Posting. © American Geophysical Union, 2019. This article is posted here by permission of American Geophysical Union for personal use, not for redistribution. The definitive version was published in Journal of Geophysical Research-Biogeosciences 124 (2019): 2823-2850, doi:10.1029/2019JG005113.
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Frontalini, F., Losada, M. T., Toyofuku, T., Tyszka, J., Golen, J., de Nooijer, L., Canonico, B., Cesarini, E., Nagai, Y., Bickmeyer, U., Ikuta, T., Tsubaki, R., Besteiro Rodriguez, C., Al Enezi, E., Papa, S., Coccioni, R., Bijma, J., & Bernhard, J. M. (2019). Foraminiferal ultrastructure: A perspective from fluorescent and fluorogenic probes. Journal of Geophysical Research-Biogeosciences, 124, 2823-2850.Related items
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