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dc.contributor.authorEstrada-Gomez, Sebastian  Concept link
dc.contributor.authorCaldas Cardoso, Fernanda  Concept link
dc.contributor.authorVargas-Muñoz, Leidy Johana  Concept link
dc.contributor.authorQuintana-Castillo, Juan Carlos  Concept link
dc.contributor.authorArenas Gómez, Claudia Marcela  Concept link
dc.contributor.authorPineda, Sandy Steffany  Concept link
dc.contributor.authorSaldarriaga-Cordoba, Monica Maria  Concept link
dc.date.accessioned2019-12-13T13:43:01Z
dc.date.available2019-12-13T13:43:01Z
dc.date.issued2019-08-27
dc.identifier.citationEstrada-Gomez, S., Caldas Cardoso, F., Johana Vargas-Munoz, L., Carlos Quintana-Castillo, J., Arenas Gomez, C. M., Steffany Pineda, S., & Maria Saldarriaga-Cordoba, M. (2019). Venomic, transcriptomic, and bioactivity analyses of Pamphobeteus verdolaga venom reveal complex disulfide-rich peptides that modulate calcium channels. Toxins, 11(9), 496.en_US
dc.identifier.urihttps://hdl.handle.net/1912/25017
dc.description© The Author(s), 2019. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Estrada-Gomez, S., Caldas Cardoso, F., Johana Vargas-Munoz, L., Carlos Quintana-Castillo, J., Arenas Gomez, C. M., Steffany Pineda, S., & Maria Saldarriaga-Cordoba, M. Venomic, transcriptomic, and bioactivity analyses of Pamphobeteus verdolaga venom reveal complex disulfide-rich peptides that modulate calcium channels. Toxins, 11(9), (2019): 496, doi:10.3390/toxins11090496.en_US
dc.description.abstractPamphobeteus verdolaga is a recently described Theraphosidae spider from the Andean region of Colombia. Previous reports partially characterized its venom profile. In this study, we conducted a detailed analysis that includes reversed-phase high-performance liquid chromatography (rp-HPLC), calcium influx assays, tandem mass spectrometry analysis (tMS/MS), and venom-gland transcriptome. rp-HPLC fractions of P. verdolaga venom showed activity on CaV2.2, CaV3.2, and NaV1.7 ion channels. Active fractions contained several peptides with molecular masses ranging from 3399.4 to 3839.6 Da. The tMS/MS analysis of active fraction displaying the strongest activity to inhibit calcium channels showed sequence fragments similar to one of the translated transcripts detected in the venom-gland transcriptome. The putative peptide of this translated transcript corresponded to a toxin, here named ω-theraphositoxin-Pv3a, a potential ion channel modulator toxin that is, in addition, very similar to other theraphositoxins affecting calcium channels (i.e., ω-theraphotoxin-Asp1a). Additionally, using this holistic approach, we found that P. verdolaga venom is an important source of disulfide-rich proteins expressing at least eight superfamilies.en_US
dc.publisherMDPIen_US
dc.relation.urihttps://doi.org/10.3390/toxins11090496
dc.rightsAttribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjecttheraphosidaeen_US
dc.subjectPamphobeteusen_US
dc.subjectpeptidesen_US
dc.subjectdisulfide-rich peptide (DRP)en_US
dc.subjectinhibitory cysteine knot (ICK)en_US
dc.subjectvenomicsen_US
dc.subjecttranscriptomeen_US
dc.subjection channelsen_US
dc.titleVenomic, transcriptomic, and bioactivity analyses of Pamphobeteus verdolaga venom reveal complex disulfide-rich peptides that modulate calcium channelsen_US
dc.typeArticleen_US
dc.identifier.doi10.3390/toxins11090496


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Attribution 4.0 International
Except where otherwise noted, this item's license is described as Attribution 4.0 International