• Login
    About WHOAS
    View Item 
    •   WHOAS Home
    • Woods Hole Oceanographic Institution
    • Academic Programs
    • WHOI Theses
    • View Item
    •   WHOAS Home
    • Woods Hole Oceanographic Institution
    • Academic Programs
    • WHOI Theses
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of WHOASCommunities & CollectionsBy Issue DateAuthorsTitlesKeywordsThis CollectionBy Issue DateAuthorsTitlesKeywords

    My Account

    LoginRegister

    Statistics

    View Usage Statistics

    Zebrafish cardiovascular cDNA microarrays : expression profiling and gene discovery in embryos exposed to 2,3,7,8-Tetrachlorodibenzo-ρ-dioxin

    Thumbnail
    View/Open
    Handley_thesis.pdf (19.76Mb)
    Date
    2003-09
    Author
    Handley, Heather Martin  Concept link
    Metadata
    Show full item record
    Citable URI
    https://hdl.handle.net/1912/2461
    DOI
    10.1575/1912/2461
    Keyword
     Zebra danio; Embryos; Tetrachlorodibenzodioxin; Toxicology; Environmental aspects 
    Abstract
    2,3,7,8-Tetrachlorodibenzo-ρ-dioxin (TCDD) is a potent teratogen that impacts the developing cardiovascular system. Hallmarks of embryonic exposure include cardiac malformation, impaired circulation, loss of erythrocytes, pericardial and yolk sac edema, and early life stage mortality. However, the mechanism of TCDD cardiovascular embryotoxicity is poorly understood. The primary goal of this thesis was to identify TCDD-responsive genes likely to be involved in processes of toxicity. We constructed microarrays using cDNA libraries derived from zebrafish embryonic and adult heart tissue. Embryonic heart arrays were used for protocol development. The resulting workflow was employed in the production of adult heart microarrays containing ~2800 unique cardiovascular genes. These arrays were used to establish gene expression profiles of zebrafish embryos exposed to 1.84±0.42 or 10.74±0.38 ng TCDD/g embryo. Alterations in cardiovascular gene expression were limited; 44 genes or ESTs were significantly differentially expressed ≥1.8-fold (p-values ≤5x10-4), and only CYP1A and CYP1B1 were induced >4- fold. Transcriptional responses to TCDD were highly dose-dependent, and adaptive responses were a prevalent feature of TCDD-modulated gene expression. Microarray analyses indicated induction of genes in three major functional classes - xenobiotic detoxification, sarcomere structure, and energy transfer. TCDD-modulation of selected genes was verified by RT-PCR. Induction of mitochondrial electron transfer genes was variable and modest; such induction provides a possible pathway to reactive oxygen generation and cardiac pathology. Sarcomere genes were generally robustly induced, but RT-PCR indicated suppression of cardiac troponin T2. The current data suggest that TCDD causes cardiomyopathy in zebrafish embryos. Investigation of a TCDD-induced EST cluster led to the discovery of a novel retroelement, EZR1. EZR1 elements lack genes necessary for autonomous retrotransposition, but are highly expressed in normal and TCDD-exposed cardiac tissue. Putative regulatory elements in LTR sequences may account for observed expression patterns. The function, if any, of EZR1 remains open to speculation.
    Description
    Submitted in partial fulfillment of the requirements for the degree of Doctor of Philosophy at the Massachusetts Institute of Technology and the Woods Hole Oceanographic Institution September 2003
    Collections
    • Biology
    • WHOI Theses
    Suggested Citation
    Thesis: Handley, Heather Martin, "Zebrafish cardiovascular cDNA microarrays : expression profiling and gene discovery in embryos exposed to 2,3,7,8-Tetrachlorodibenzo-ρ-dioxin", 2003-09, DOI:10.1575/1912/2461, https://hdl.handle.net/1912/2461
     

    Related items

    Showing items related by title, author, creator and subject.

    • Thumbnail

      Physiological and behavioral responses, and their variability, in squid, Doryteuthis pealeii, embryos and paralarvae reared under chronic ocean acidification 

      Zakroff, Casey (Massachusetts Institute of Technology and Woods Hole Oceanographic Institution, 2019-09)
      Ocean acidification (OA) and related stressors, like warming, are occurring rapidly in coastal systems. There is concern about the impacts these stressors may have on the early development of species that use the nearshore ...
    • Thumbnail

      Data on chorion thickness from embryos studied in an experiment on CO2 sensitivity of Northern sand lance (Ammodytes dubius) embryos conducted in 2018 

      Baumann, Hannes; Nye, Janet (Biological and Chemical Oceanography Data Management Office (BCO-DMO). Contact: bco-dmo-data@whoi.edu, 2022-01-13)
      Source data of two years of experimentation on Northern sand lance (Ammodytes dubius) embryos at different temperature and pCO2 conditions. Founder adults were sampled at Stellwagen Bank National Marine Sanctuary (SBNMS) ...
    • Thumbnail

      Data on unhatched embryos from an experiment on CO2 sensitivity of Northern sand lance (Ammodytes dubius) embryos conducted in 2018 

      Baumann, Hannes; Nye, Janet (Biological and Chemical Oceanography Data Management Office (BCO-DMO). Contact: bco-dmo-data@whoi.edu, 2022-01-13)
      Source data of two years of experimentation on Northern sand lance (Ammodytes dubius) embryos at different temperature and pCO2 conditions. Founder adults were sampled at Stellwagen Bank National Marine Sanctuary (SBNMS) ...
    All Items in WHOAS are protected by original copyright, with all rights reserved, unless otherwise indicated. WHOAS also supports the use of the Creative Commons licenses for original content.
    A service of the MBLWHOI Library | About WHOAS
    Contact Us | Send Feedback | Privacy Policy
    Core Trust Logo