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    The Chlamydomonas flagellar membrane glycoprotein FMG-1B is necessary for expression of force at the flagellar surface

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    Article_and_Supplementary Material (7.479Mb)
    Date
    2019-08-19
    Author
    Bloodgood, Robert A.  Concept link
    Tetreault, Joseph  Concept link
    Sloboda, Roger D.  Concept link
    Metadata
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    Citable URI
    https://hdl.handle.net/1912/24526
    As published
    https://doi.org/10.1242/jcs.233429
    DOI
    10.1242/jcs.233429
    Keyword
     Chlamydomonas; FMG-1B; Flagella; Cilia; Flagellar membrane; Gliding motility; Surface motility 
    Abstract
    In addition to bend propagation for swimming, Chlamydomonas cells use their flagella to glide along a surface. When polystyrene microspheres are added to cells, they attach to and move along the flagellar surface, thus serving as a proxy for gliding that can be used to assay for the flagellar components required for gliding motility. Gliding and microsphere movement are dependent on intraflagellar transport (IFT). Circumstantial evidence suggests that mechanical coupling of the IFT force-transducing machinery to a substrate is mediated by the flagellar transmembrane glycoprotein FMG-1B. Here, we show that cells carrying an insertion in the 5′-UTR of the FMG-1B gene lack FMG-1B protein, yet assemble normal-length flagella despite the loss of the major protein component of the flagellar membrane. Transmission electron microscopy shows a complete loss of the glycocalyx normally observed on the flagellar surface, suggesting it is composed of the ectodomains of FMG-1B molecules. Microsphere movements and gliding motility are also greatly reduced in the 5′-UTR mutant. Together, these data provide the first rigorous demonstration that FMG-1B is necessary for the normal expression of force at the flagellar surface in Chlamydomonas. This article has an associated First Person interview with authors from the paper.
    Description
    Author Posting. © Company of Biologists, 2019. This article is posted here by permission of Company of Biologists for personal use, not for redistribution. The definitive version was published in Journal of Cell Science 132 (2019): jcs.233429, doi:10.1242/jcs.233429.
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    Suggested Citation
    Bloodgood, R. A., Tetreault, J., & Sloboda, R. D. (2019). The Chlamydomonas flagellar membrane glycoprotein FMG-1B is necessary for expression of force at the flagellar surface. Journal of Cell Science, 132, jcs.233429.
     

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