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dc.contributor.authorNagashima, Ryosuke  Concept link
dc.contributor.authorHibino, Kayo  Concept link
dc.contributor.authorAshwin, S. S.  Concept link
dc.contributor.authorBabokhov, Michael  Concept link
dc.contributor.authorFujishiro, Shin  Concept link
dc.contributor.authorImai, Ryosuke  Concept link
dc.contributor.authorNozaki, Tadasu  Concept link
dc.contributor.authorTamura, Sachiko  Concept link
dc.contributor.authorTani, Tomomi  Concept link
dc.contributor.authorKimura, Hiroshi  Concept link
dc.contributor.authorShribak, Michael  Concept link
dc.contributor.authorKanemaki, Masato T.  Concept link
dc.contributor.authorSasai, Masaki  Concept link
dc.contributor.authorMaeshima, Kazuhiro  Concept link
dc.date.accessioned2019-07-08T16:37:49Z
dc.date.available2019-07-08T16:37:49Z
dc.date.issued2019-03-01
dc.identifier.citationMBL Nagashima, R., Hibino, K., Ashwin, S. S., Babokhov, M., Fujishiro, S., Imai, R., Nozaki, T., Tamura, S., Tani, T., Kimura, H., Shribak, M., Kanemaki, M. T., Sasai, M., & Maeshima, K. (2019). Single nucleosome imaging reveals loose genome chromatin networks via active RNA polymerase II. Journal of Cell Biology, 218(5), 1511-1530.en_US
dc.identifier.urihttps://hdl.handle.net/1912/24331
dc.description© The Author(s), 2019. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Nagashima, R., Hibino, K., Ashwin, S. S., Babokhov, M., Fujishiro, S., Imai, R., Nozaki, T., Tamura, S., Tani, T., Kimura, H., Shribak, M., Kanemaki, M. T., Sasai, M., & Maeshima, K. Single nucleosome imaging reveals loose genome chromatin networks via active RNA polymerase II. Journal of Cell Biology, 218(5), (2019):1511-1530, doi:10.1083/jcb.201811090.en_US
dc.description.abstractAlthough chromatin organization and dynamics play a critical role in gene transcription, how they interplay remains unclear. To approach this issue, we investigated genome-wide chromatin behavior under various transcriptional conditions in living human cells using single-nucleosome imaging. While transcription by RNA polymerase II (RNAPII) is generally thought to need more open and dynamic chromatin, surprisingly, we found that active RNAPII globally constrains chromatin movements. RNAPII inhibition or its rapid depletion released the chromatin constraints and increased chromatin dynamics. Perturbation experiments of P-TEFb clusters, which are associated with active RNAPII, had similar results. Furthermore, chromatin mobility also increased in resting G0 cells and UV-irradiated cells, which are transcriptionally less active. Our results demonstrated that chromatin is globally stabilized by loose connections through active RNAPII, which is compatible with models of classical transcription factories or liquid droplet formation of transcription-related factors. Together with our computational modeling, we propose the existence of loose chromatin domain networks for various intra-/interchromosomal contacts via active RNAPII clusters/droplets.en_US
dc.description.sponsorshipWe thank Dr. Y. Hiromi, Dr. S. Hirose, Dr. H. Seino, and Dr. S. Ide for critical reading of this manuscript. We thank Dr. S. Ide, Dr. D. Kaida, Dr. T. Nagai, Dr. V. Doye, Dr. G. Felsenfeld, and Dr. K. Horie for valuable help and materials. We also thank the Maeshima laboratory members for helpful discussions and support. R. Imai and T. Nozaki are Japan Society for the Promotion of Science Fellows. R. Nagashima was supported by 2017 SOKENDAI Short-Stay Study Abroad Program. This work was supported by a Japan Society for the Promotion of Science grant (16H04746), Takeda Science Foundation, RIKEN Pioneering Project, a Japan Science and Technology Agency Core Research for Evolutional Science and Technology grant (JPMJCR15G2), a National Institute of General Medical Sciences grant (R01-GM101701), and National Institute of Genetics JOINT (2016-A2 (6)).en_US
dc.publisherRockefeller University Pressen_US
dc.relation.urihttps://doi.org/10.1083/jcb.201811090
dc.rightsAttribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.titleSingle nucleosome imaging reveals loose genome chromatin networks via active RNA polymerase II.en_US
dc.typeArticleen_US
dc.identifier.doi10.1083/jcb.201811090


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Attribution 4.0 International
Except where otherwise noted, this item's license is described as Attribution 4.0 International