Quantitative orientation-independent differential interference contrast (DIC) microscopy coupled with orientation-independent Polarization microscopy
LaFountain, James R.
MetadataShow full item record
Differential interference contrast (DIC) microscopy is widely used to observe structure and motion in unstained, transparent living cells and isolated organelles, producing a monochromatic shadowcast image of optical phase gradient. Polarized light microscopy (Pol) reveals structural anisotropy due to form birefringence, intrinsic birefringence, stress birefringence, etc. DIC and Pol complement each other as, for example, in a live dividing cell, the DIC image will clearly show the chromosomes while the Pol image will depict the distribution of the birefringent microtubules in the spindle. Both methods, however, have the same shortcomings: they require the proper orientation of a specimen in relation to the optical system in order to achieve best results.
Author Posting. © Microscopy Society of America, 2007. This article is posted here by permission of Cambridge University Press for personal use, not for redistribution. The definitive version was published in Microscopy and Microanalysis 13 Suppl. 2 (2007): 10-11, doi:10.1017/S1431927607075186.
Showing items related by title, author, creator and subject.
Mapping optical path length and image enhancement using quantitative orientation-independent differential interference contrast microscopy Shribak, Michael; Larkin, Kieran G.; Biggs, David (SPIE, 2017-01-06)We describe the principles of using orientation-independent differential interference contrast (OI-DIC) microscopy for mapping optical path length (OPL). Computation of the scalar two-dimensional OPL map is based on an ...
Shribak, Michael; Inoue, Shinya (2006)The image in a regular DIC microscope reflects the orientation of the prism shear direction and the optical path gradients in a phase specimen. If the shear direction lies parallel to the specimen boundary no contrast ...
Quantitative orientation-independent differential interference contrast microscope with fast switching shear direction and bias modulation Shribak, Michael (Optical Society of America, 2013-03-27)We describe a quantitative orientation-independent differential interference contrast (DIC) microscope, which allows bias retardation to be modulated and shear directions to be switched rapidly without any mechanical ...